Plant Seed(Large) Direct PCR Kit II-UNG(without Sampling Tools)
Kit Description:
Direct use of seed lysate as a template for PCR amplification, no separate extraction of DNA, fast and high sensitivity. The UNG anti-pollution system has been added to prevent false positives.
◮No time-consuming and expensive DNA purification is required.
◮The sample demand is small, just take a single seed.
◮No special treatments such as grinding and crushing are required, and the operation is simple.
◮The optimized PCR system enables PCR to have higher specificity and stronger tolerance to PCR reaction inhibitors.
Descriptions
This product uses a unique lysis reaction system, which can quickly release genomic DNA from plant seed samples with low polysaccharide and polyphenol content (such as: rice, wheat, tobacco, corn, soybeans, etc.) for use in PCR reactions, so it is particularly suitable Large-scale genetic testing. In order to meet the needs of different detection tests, this kit can use multiple types of samples (such as whole seeds, small tissue cuts, or ground samples of multiple seeds) for experiments. Among them, for seed samples that still need to be germinated, you can choose to cut out micro-tissue cuts (1-5mg) other than the seed embryo for experiment; for experiments that need to be sampled in a large number of samples, you can choose to mix multiple samples and combine them. After grinding into particles with a diameter of about 0.5mm, the experiment can be carried out (at least 0.1% of the target sample can be detected).
Specifications
50T,200T,500T,2000T
Kit components
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Plant Seed Direct PCR Kit II-UNG Plant Seed (Large) Direct PCR Kit-UNG |
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Kit Composition |
TP-0314T | TP-03141 |
TP-03142 |
TP-03143 | |
| 50 T | 200 T | 500 T | 2000 T | ||
|
Part I |
Buffer SP1 | 30ml | 120ml |
100ml×3 |
400ml×3 |
| Buffer SP2 | 3ml | 10ml | 25ml | 100ml | |
| 6× DNA Loading Buffer | 1.5ml | 1.5ml | 1.5ml | 1.5ml×4 | |
| Part II | 2× Seed PCR EasyTM Mix(UNG) | 500μl | 1ml×2 |
1.7ml×3 |
1.7ml×12 |
| Manual | 1 | 1 | 1 | 1 | |
Features and advantages
- No need time-consuming and expensive DNA purification is required.
- The sample demand is small, just take a single seed.
- No special treatments such as grinding and crushing are required, and the operation is simple.
- The optimized PCR system enables PCR to have higher specificity and stronger tolerance to PCR reaction inhibitors.
- Anti-pollution PCR system 2× Seed PCR EasyTM Mix (UNG) can effectively eliminate the pollution caused by PCR products and ensure the specificity and accuracy of amplification.
Workflow
Storage conditions
1.Transportation
The whole process of low-temperature ice box transportation, to ensure that the kit Part II is in a state of <4 ℃.
2.Storage
Part I of this kit should be stored at room temperature or 2-8°C.
Part II should be stored at -20°C.
