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SARS-CoV-2 Nucleic Acid Detection Kit (Multiplex PCR Fluorescent Probe Method)

Kit Description:

 

◮ Direct PCR: No need for nucleic acid extaction kit and System, completed 96 specimen in 1 hour or so.

 

◮ Low equipment requirement and highly flexible use:Only need Real-Time PCR system .

 

◮ LoD and high sensitivity:Detection sensitivity can reach the lowest level as 500 copies/ml.

foregene strength


Product Detail

Product Tags

Intended Use

This kit uses Real-time RT PCR technology (rRT-PCR) for qualitative detection of SARS-CoV-2 nucleic acids in human nasopharyngeal or oropharyngeal swab samples.

Principle of testing

This primer and probe sets are designed for the detection of the conserved sequences of SARS-CoV-2 (ORF1ab gene and N gene). The kit contains an Internal Control that is used to assess specimen quality.
Real-Time PCR technology utilizes polymerase chain reaction (PCR) for the amplification of specific target
sequences and target specific probes for the detection of the amplified RNA. The probes are labelled with
fluorescent reporter and quencher dyes.

Quick Details

Specifications: 48 Rxns/kit, 96 Rxns/kit

◮ Specifificity:96.72%

   Sensitivity:97.92%

◮Applicable specimen types
     Nasopharyngeal Swab or Oropharyngeal Swab
◮ Applicable instrument
    ABI7500, Bio-Rad CFX96, Roche LightCycler 480, SLAN-96S

 

Components

No.

Component

Amount

Main components

48 Rxns

96 Rxns

1 Nucleic acid release agent 1.4mL/tube 2 tubes 5.3 mL/bottle 1 bottle Surfactant
2 RNA protectant 27 μL/tube 1 tube 53 μL/tube 1 tube RNase inhibitor
3 SARS-CoV-2 reaction solution 800 μL/tube 1 tube 1600 μL/tube 1 tube Primer, probe, reaction buffer, dNTP
4 SARS-CoV-2 enzyme mixture 80 μL/tube 1 tube 160 μL/tube 1 tube Hot start Taq enzyme, M-MLV enzyme
5 SARS-CoV-2 positive control 100 μL/tube 1 tube 100 μL/tube 1 tube Recombinant plasmid containing target fragment, RNA
6 SARS-CoV-2 negative control 1200 μL/tube 1 tube 1200 μL/tube 1 tube TE buffer

Features&advantages

Direct PCR
No need for nucleic acid extaction kit and System, completed 96 specimen in 1 hour or so.
Low equipment requirement and highly flflexible use
Only need Real-Time PCR system.
LoD and high sensitivity
Detection sensitivity can reach the lowest level as 500 copies/ml.
amplification curve-RT-qPCR

How to use

Method 1: Direct PCR method 

Method 2: Viral RNA Isolation + PCR 

Method 1: Direct PCR method   using Sample Release Agent  

 

Detection procedure

Operation Procedures

Procedures

Cold Chain Transportation and Storage

Sealed from light and stored at -20±5℃;

storage
storage2

Shelf life

1 year

Ordering Information

Ordering information

Quick Operation video


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