SARS-CoV-2 Nucleic Acid Detection Kit (Multiplex PCR Fluorescent Probe Method)

SARS-CoV-2 Nucleic Acid Detection Kit (Multiplex PCR Fluorescent Probe Method)

Kit Description:

This kit uses Real-time RT PCR technology(rRT-PCR) for qualitative detection of SARS-CoV-2 (ORF1ab gene and N gene) nucleic acids in human nasopharyngeal or oropharyngeal swab samples, by multiplex PCR fluorescent probe method.This kit contains an internal control to assess specimen quality, thus no need for nucleic acid purification, can complete test in 1 hour, especially suitable for large-scale rapid detection.

20+ years R&D Manufacturing Experience         25+ highest impact factor                       

1000+ different lab reagents                                100+ universities partner                         

400+ distributors                                                   2000+ SCI citations of the lab reagents 

Product Detail

Product Tags


48 Rxns, 96 Rxns




Main components

48 Rxns

96 Rxns

1 Nucleic acid release agent 1.4mL/tube 2 tubes 5.3 mL/bottle 1 bottle Surfactant
2 RNA protectant 27 μL/tube 1 tube 53 μL/tube 1 tube RNase inhibitor
3 SARS-CoV-2 reaction solution 800 μL/tube 1 tube 1600 μL/tube 1 tube Primer, probe, reaction buffer, dNTP
4 SARS-CoV-2 enzyme mixture 80 μL/tube 1 tube 160 μL/tube 1 tube Hot start Taq enzyme, M-MLV enzyme
5 SARS-CoV-2 positive control 100 μL/tube 1 tube 100 μL/tube 1 tube Recombinant plasmid containing target fragment, RNA
6 SARS-CoV-2 negative control 1200 μL/tube 1 tube 1200 μL/tube 1 tube TE buffer


Quick and easy.
Avoid nucleic acid loss.
Low detection limit and high sensitivity.
Low equipment requirement and highly flexible use.

Detection sensitivity can reach the lowest level as 500 copies/mL).

GCE/mL Ct value
N gene ORF1ab gene
2.00E+08 16.43 15.16
2.00E+07 19.48 18.58
2.00E+06 22.71 21.86
2.00E+05 25.95 25.33
2.00E+04 29.30 28.49
2.00E+03 32.37 31.37
2.00E+02 35.32 34.47
2.00E+01 38.29 38.27
2.00E+00 N/A N/A
2.00E-01 N/A N/A

Linear amplification curve

amplification curve-RT-qPCR

How to use

1. Applicable instrument

covid-19 nucleic acid detection kit1

2. Detection procedure

covid-19 nucleic acid detection kit2

Sample processing:Naopharyngeal or oropharyngeal swab sample (only 1 step operation)(Wait for 10 mins)

PCR amplificaton(<=55min)


covid-19 nucleic acid detection kit4


sealed from light and stored at -20±5℃;


Shelf life: 1 year

  • Previous:
  • Next:

  • Write your message here and send it to us