Animal Tissue Direct PCR kit (without Sampling Tools) Protocol
Kit Description:
Directly use animal tissue lysate as a template for PCR amplification, without separate extraction of DNA, fast and high sensitivity.
◮No time-consuming and expensive DNA purification is required.
◮The sample demand is small, just take a single seed.
◮No special treatments such as grinding and crushing are required, and the operation is simple.
◮The optimized PCR system enables PCR to have higher specificity and stronger tolerance to PCR reaction inhibitors.
Descriptions
This kit uses a unique lysis buffer system to quickly release genomic DNA from animal tissue samples for PCR reactions, so it is particularly suitable for large-scale genetic testing.
The process of releasing genomic DNA from the lysis buffer is completed within 10-30 minutes at 65°C. No other processes such as protein and RNA removal are required, and the released trace DNA can be used as a template for PCR reaction.
2× PCR EasyTM Mix has strong tolerance to PCR reaction inhibitors, and can use the lysate of the sample to be tested as a template for efficient and specific amplification. This reagent contains Foregene D-Taq DNAPolymerase, dNTPs, MgCl2, reaction buffer, PCR optimizer and stabilizer.
D-Taq DNA polymerase is a DNA polymerase specially developed by Foregene for direct PCR reactions. D-Taq DNA polymerase has strong tolerance to a variety of PCR reaction inhibitors, and can efficiently amplify trace amounts of DNA in various complex reaction systems, and the amplification speed can reach 2Kb/min, which is especially suitable for Direct PCR reaction.
Specifications
50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns
Kit components
|
Part I |
Buffer AL |
|
Foregene Protease |
|
|
6× DNA Loading Buffer |
|
|
Part II |
2× PCR EasyTM Mix |
Features&advantages
■ No time-consuming and expensive DNA purification
■ The sample demand is small, as little as 5mg animal tissue can be tested.
■ No special treatment such as grinding and crushing is required, and the operation is simple.
■ Optimized PCR system makes PCR have higher specificity and stronger tolerance to PCR reaction inhibitors.
Kit parameters
Application: a variety of animal tissues.
DNA released from sample lysis: only used as a PCR template.
The kit can be used for the following purposes: identification of transgenosis, animal genotyping, etc.
Work flow
Storage and Shelf life
Part I of this kit should be stored at 2-8℃.
The reagent Buffer AL can be stored for 12 months under dry conditions; it can be stored at 2-8℃ for longer storage.
Reagent Foregene Protease has a unique formula. To ensure its activity and stability, please store it at 4℃ for 12 months.
Reagent 6×DNA Loading Buffer can be stored at 4℃ or -20℃ for a long time.
Part II of this kit should be stored at -20℃ .
Reagent 2×PCR EasyTM Mix can be stored for 12 months at -20℃ ; if it is used frequently, it can also be stored at 4℃ for short-term storage (use up within 10 days).
