Animal Tissue Direct PCR kit

Kit Description:

No time-consuming and expensive DNA purification

The sample demand is small, as little as 5mg animal tissue can be tested.

No special treatment such as grinding and crushing is required, and the operation is simple.

Optimized PCR system makes PCR have higher specificity and stronger tolerance to PCR reaction inhibitors.

foregene strength


Product Detail

Product Tags

Descriptions

This kit uses a unique lysis buffer system to quickly release genomic DNA from animal tissue samples for PCR reactions, so it is particularly suitable for large-scale genetic testing.

The process of releasing genomic DNA from the lysis buffer is completed within 10-30 minutes at 65°C. No other processes such as protein and RNA removal are required, and the released trace DNA can be used as a template for PCR reaction.

2× PCR EasyTM Mix has strong tolerance to PCR reaction inhibitors, and can use the lysate of the sample to be tested as a template for efficient and specific amplification. This reagent contains Foregene D-Taq DNAPolymerase, dNTPs, MgCl2, reaction buffer, PCR optimizer and stabilizer.

D-Taq DNA polymerase is a DNA polymerase specially developed by Foregene for direct PCR reactions. D-Taq DNA polymerase has strong tolerance to a variety of PCR reaction inhibitors, and can efficiently amplify trace amounts of DNA in various complex reaction systems, and the amplification speed can reach 2Kb/min, which is especially suitable for Direct PCR reaction.

Specifications

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Kit components

Part I

Buffer AL

Foregene Protease

6× DNA Loading Buffer

Part II

2× PCR EasyTM Mix

Features&advantages

■  No time-consuming and expensive DNA purification

■ The sample demand is small, as little as 5mg animal tissue can be tested.

■ No special treatment such as grinding and crushing is required, and the operation is simple.

■ Optimized PCR system makes PCR have higher specificity and stronger tolerance to PCR reaction inhibitors.

Kit parameters

Application: a variety of animal tissues.

DNA released from sample lysis: only used as a PCR template.

The kit can be used for the following purposes: identification of transgenosis, animal genotyping, etc.

Work flow

wk flw-animal tissue direct PCR

Storage and Shelf life

Part I of this kit should be stored at 2-8.

The reagent Buffer AL can be stored for 12 months under dry conditions; it can be stored at 2-8℃  for longer storage.

 Reagent Foregene Protease has a unique formula. To ensure its activity and stability, please store it at 4for 12 months.

 Reagent 6×DNA Loading Buffer can be stored at 4or -20for a long time.

Part II of this kit should be stored at -20.

 Reagent 2×PCR EasyTM Mix can be stored for 12 months at -20; if it is used frequently, it can also be stored at 4for short-term storage (use up within 10 days).


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