Blood DNA Mini Kit
Kit Description:
Quickly purify high-quality genomic DNA from anticoagulated blood (<1ml).
◮ No RNase contamination: The DNA-Only Column provided by the kit makes it possible to remove the RNA from the genomic DNA without adding RNase during the experiment, preventing the laboratory from being contaminated by exogenous RNase.
◮ Fast speed: Foregene Protease has higher activity than similar proteases, and digests tissue samples quickly; the operation is simple, and the genomic DNA extraction operation can be completed within 20-80 minutes.
◮ Convenient: The centrifugation is performed at room temperature, and there is no need for 4°C low-temperature centrifugation or ethanol precipitation of DNA.
◮ Safety: No organic reagent extraction is required.
◮ High quality: The extracted genomic DNA has large fragments, no RNA, no RNase, and extremely low ion content, which can meet the requirements of various experiments.
◮ Micro-elution system: It can increase the concentration of genomic DNA, which is convenient for downstream detection or experiment.
Kit Descriptions
This kit adopts new Foregene Protease Plus and unique BL1 and BL2 buffer systems, which can completely digest anticoagulant blood samples in a short period of time, thus avoiding DNA degradation to the maximum degree and obtaining the maximum amount of genomic DNA. The rapid blood processing system and the simple DNA-Only column operation greatly simplify the extraction of blood genome, making the genomic DNA can be obtained of high quality and high purity within 40 minutes.
The DNA-only silica gel membrane used in the centrifuge column of this kit is a new type of material unique to our company, which can bind DNA efficiently and specifically, and can remove RNA, impurities, proteins, ions and other organic compounds in cells to the maximum extent. The obtained DNA fragments are large, high purity, stable and reliable quality. The maximum carrying capacity of a DNA-Only column is 80 μg DNA. The obtained DNA can be used in molecular biology experiments such as enzyme digestion, PCR, Southern hybridization and library construction.
The kit can process up to 1ml of blood at a time to obtain a sufficient amount of high-purity genomic DNA.
Specifications
|
Blood DNA Mini Kit |
|||
|
Kit contents |
DE-05111 |
DE-05112 |
DE-05113 |
|
50 T |
100 T |
250 T |
|
|
Buffer BL1 |
15 mL |
30 ml |
75 ml |
|
Buffer BL2 * |
15 mL |
30 ml |
75 ml |
|
Buffer DC |
100 mL |
200 ml |
500 ml |
|
Buffer PW * |
25 mL |
50 ml |
125 ml |
|
Buffer WB1 |
15 mL |
30 ml |
75 ml |
|
Buffer EB |
10 mL |
20 ml |
50 ml |
|
Foregene Protease Plus |
1.25 mL |
2.5 ml |
6.5 ml |
|
DNA-Only Column |
50 |
100 |
250 |
|
Manual |
1 |
1 |
1 |
*: Buffer BL2 and Buffer PW contain irritating liquid salt. Please wear gloves and take relevant protective measures when operating.
Features&advantages
-No RNase contamination: The DNA-Only Column provided by the kit makes it possible to remove RNA from genomic DNA without adding RNase during the experiment, avoiding the laboratory from being contaminated by exogenous RNase.
-Fast speed: Foregene Protease Plus has higher activity than similar proteases, and digests tissue samples faster; the operation is simple, and the genomic DNA extraction operation can be completed within 40 minutes.
-Convenience: The centrifugation is performed at room temperature, 4°C centrifugation or ethanol precipitation of DNA is not required.
-Safety: No organic reagent extraction is used.
-High quality: The extracted genomic DNA fragments are large, no RNA, no RNase, and extremely low ion content, which can meet the requirements of various experiments.
Kit application
It is suitable for extraction and purification of genomic DNA from fresh or frozen anticoagulated whole blood.
Work flow
Storage and shelf life
This kit can be stored for 12 months under dry conditions at room temperature (15-25°C); if it needs to be stored for a longer period of time, it can be stored at 2-8°C.
Note: If stored at low temperature, the solution is prone to precipitation. Before use, be sure to place the solution in the kit at room temperature for a period of time. If necessary, preheat it in a 37°C water bath for 10 minutes to dissolve the precipitate, and mix it before use.
The Foregene Protease Plus solution has a unique formula, which is active when stored at room temperature for a long time (3 months); it will be more active and stable when stored at 4°C, so it is recommended to store it at 4°C, remember not to keep it at -20°C.
