Blood SuperDirectᵀᴹ PCR Kit-Heparin Blood Direct PCR Master Mix for Genotyping of Blood
Kit Description:
Directly use heparin anticoagulated whole blood as a template for PCR amplification, with high sensitivity and high amplification efficiency.
◮No need for time-consuming and expensive DNA purification, no pretreatment, and direct use of whole blood as a PCR template.
◮The system has strong amplification ability and can sensitively detect the genome and foreign target DNA fragments in the blood.
◮2× SuperEasyTM Mix-Heparin High blood tolerance: up to 45% in human blood and 20% in mouse blood.
◮The sample is fully enclosed, so there is no need to worry about sample contamination and false positive PCR results.
Specifications
200 ×20μl rxns, 2000 ×20μl rxns
2× SuperEasyTM Mix-Heparin：Contains the specially modified D-Taq DNA Polymerase, dNTPs, MgCl2, reaction buffer, PCR reaction enhancer, optimizer, stabilizer, etc. In the PCR reaction, just add the appropriate anticoagulated whole blood, primers, and ddH2O to the corresponding 2× SuperEasyTM Mix-Heparin to be used in the PCR reaction.
6× DNA Loading Buffer：The Loading Buffer does not contain SDS. It is recommended to use the 6× DNA Loading Buffer included with the kit when performing agarose gel electrophoresis in order to obtain good electrophoresis results. Do not use Loading Buffer containing SDS, otherwise there will be a large tail of bright light in the lane during electrophoresis, which will affect the experimental results.
Kit components
| 2× SuperEasyTM Mix-Heparin |
| 6× DNA Loading Buffer |
|
Instructions |
Features&advantages
-No need for time-consuming and expensive DNA purification, no pretreatment, and direct use of whole blood as a PCR template.
-The system has strong amplification ability and can sensitively detect the genome and foreign target DNA fragments in the blood.
-2× SuperEasyTM Mix-Heparin High blood tolerance: up to 45% in human blood and 20% in mouse blood.
-The sample is fully enclosed, so there is no need to worry about sample contamination and false positive PCR results.
Kit application
Dedicated to direct PCR identification of Heparin anti-coagulated whole blood. (Including: human blood, mouse blood, chicken blood, bird blood, cow blood, dog blood, etc.)
Workflow
Storage and Shelf life
This kit should be stored at -20℃。
2× SuperEasyTM Mix-Heparin，If used frequently, it can be stored at 4°C for short-term storage (use up within 10 days).
6× DNA Loading Buffer,It can be stored at 4°C or -20°C for a long time.
