Cell Total RNA Isolation Kit

Kit Description:

RNase-Free

Operated at room temperature (15-25℃)

With DNA-Cleaning Column

High RNA yield

Fast:Finish extraction in 11 minutes

Safety:None Organic chemical 

foregene strength


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  • Product Detail

    Product Tags

    Description

    50 Preps, 200 Preps

    This kit uses the spin column and formula developed by Foregene, which can efficiently extract high-purity and high-quality total RNA from cells cultured in 96, 24, 12, and 6-well plates.

    The kit provides an efficient DNA-Cleaning Column, which can easily separate the supernatant and cell lysate, bind and remove genomic DNA. The operation is simple and time-saving.

    The RNA-only Column can efficiently bind RNA with a unique formula. A large number of samples can be processed simultaneously.

    Kit components

    Kit composition RE-03111 RE-03114
    50 T 200 T
    Buffer cRL1* 25 mL 100 mL
    Buffer cRL2 15 mL 60 mL
    Buffer RW1* 25 mL 100 mL
    Buffer RW2 24 mL 96 mL
    RNase-Free ddH2O 10 mL 40 mL
    RNA-Only Column 50 200
    DNA-Cleaning Column 50 200
    Instruction 1 1

    *Please wear gloves and take protective measures during the operation as Buffer cRL1 and Buffer RW1 contain irritating chaotropic salts.

    Features&advantages

    ■ The whole process is operated at room temperature (15-25℃), without ice bath and low temperature centrifugation.
    ■ The whole kit is RNase-Free, no need to worry about RNA degradation.
    ■ DNA-Cleaning Column specifically binds DNA, so that the kit can remove genomic DNA contamination without adding additional DNase.
    ■ High RNA yield: RNA-only Column and unique formula can efficiently purify RNA.
    ■ Fast speed: easy to operate and can be completed in 11 minutes.
    ■ Safety: No organic reagent is required.
    ■ High quality: The purified RNA is of high purity, free of protein and other impurities, and can meet various subsequent experiments.

    123

    Kit application

    It is suitable for extraction and purification of total RNA from cultured cells in 96, 24, 12, and 6-well plates.

    Work flow

    cell total RNA

    Diagram

    Cell Total RNA Isolation Kit Work Flow1

    The agarose gel battery diagram of Cell Total RNA Isolation Kit treated the above different numbers of cells, 20μl volume elution, take 2μl purified total RNA 1%.

    Storage and shelf life

    The kit can be stored for 12 months at room temperature (15–25 ℃) or 2–8 ℃ for longer time(24 months).

    Buffer cRL1 can be stored at 4 ℃ for 1 month after adding 2-hydroxy-1-ethanethiol(optional).


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