EndoFree Maxi Plasmid Kit (Spin Column)
Kit Description:
The whole extraction process takes only 1 hour, ensuring the convenient and quick operation.
Description
The EndoFree Maxi Plasmid Kit adopts a unique silica membrane adsorption technique to efficiently and specifically bind plasmid DNA. By combining the special Endotoxin Removal Buffer P4 and EndoFree Maxi Filtration Columns, endotoxin, proteins and other impurities can be effectively removed. The whole extraction process takes only 1 hour, ensuring the convenient and quick operation.
Recommended bacteria amount for each practice: For high-copy plasmid, it is recommended to use 100 ml of bacteria culture media, and the yield from which is generally around 500-1500 μg. For low-copy plasmid, 200 ml bacteria culture media is recommended to generate around 200-600 μg plasmids.
Kit components
| Product Components |
D909-E（10 preps） |
| Buffer BL | 30 ml |
| Buffer P1 | 100 ml |
| Buffer P2 | 100 ml |
| Buffer P4 | 100 ml |
| Buffer DW2 | 44 ml |
| Buffer EB | 30 ml |
| RNase A(10 mg/ml) | 1 ml |
| DNAPure Maxi Spin Columns | 10 pcs |
| FinePure Maxi Filter Syringe | 10 pcs |
| 50 ml Collection Tubes | 10 pcs |
Detection of Plasmid DNA Concentration and Purity
The purity and concentration of the extracted plasmid DNA can be detected by agarose gel electrophoresis and an ultraviolet spectrophotometer. An OD260 value of 1 corresponds to approximately 50 μg/ml of double stranded DNA. The OD260/OD280 value of the purified plasmid DNA is generally in the range of 1.7-1.9. The purified plasmid DNA can be directly applied to experiments with high purity requirements, such as cell transfection or in vivo experiments.
Kit application
The plasmid DNA extracted by this kit can be applied to various routine operations, including enzymatic digestion, PCR, sequencing, ligation, transformation as well as the transfection for various cell types.
Storage and Shelf life
EndoFree Maxi Plasmid Kit can be stored at room temperature (15-25°C) under dry conditions for up to 12 months. Add RNase A to Buffer P1 before the first use, mix well and store at 2-8°C. The mix can be stored stably for more than 6 months.
