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Water DNA Isolation Kit DNA Extraction and Purification Kit for Water

Kit Description:

 Quickly purify high-quality genomic DNA from microorganisms in various water samples.

◮ No RNase contamination: The DNA-Only Column provided by the kit makes it possible to remove the RNA from the genomic DNA without adding RNase during the experiment, preventing the laboratory from being contaminated by exogenous RNase.

 Fast speed: Foregene Protease has higher activity than similar proteases, and digests tissue samples quickly; the operation is simple, and the genomic DNA extraction operation can be completed within 20-80 minutes.

◮ Convenient: The centrifugation is performed at room temperature, and there is no need for 4°C low-temperature centrifugation or ethanol precipitation of DNA.

◮ Safety: No organic reagent extraction is required.

◮ High quality: The extracted genomic DNA has large fragments, no RNA, no RNase, and extremely low ion content, which can meet the requirements of various experiments.

◮ Micro-elution system: It can increase the concentration of genomic DNA, which is convenient for downstream detection or experiment.

foregene strength


Product Detail

Product Tags

Specifications

50 Preps

-Lysozyme: enzymatically hydrolyze the cell wall of positive bacteria.

-Buffer TE: used to prepare 100mg/ml Lysozyme solution and provide lysozyme enzymatic hydrolysis environment.

-Buffer SG1 & Buffer SG2: Provide sample protease digestion environment.

-Foregene Protease: Enzymatically hydrolyze samples in a protease enzymatic environment to release genomic DNA.

-Buffer SG3: Inactivate Foregene Protease and provide a DNA loading environment.

-Buffer SG4: Supplement to provide DNA loading environment.

-Buffer PW: Remove impurities such as protein and RNA in DNA.

-Buffer WB: Remove residual salt ions in DNA.

-Buffer EB: elute the DNA on the purification column membrane.

-DNA-only Column: specifically adsorb the genomic DNA in the lysate.

Kit components

Buffer SG1

 Buffer SG2
 Buffer SG3
 Buffer SG4
 Buffer PW
 Buffer WB
 Buffer EB
 Buffer TE
 Foregene Protease
 Lysozyme
 DNA-Only Column

Instructions

Features&advantages

-No RNase contamination: The DNA-Only Column provided by the kit makes it possible to remove RNA from genomic DNA without additional RNase during the experiment, avoiding the laboratory from being contaminated by exogenous RNase.

-Fast speed: The operation is simple, and the water genomic DNA extraction operation can be completed within 40 minutes.

-Convenient: The centrifugation is performed at room temperature, and there is no need for 4°C low-temperature centrifugation or ethanol precipitation of DNA.

-Safety: no need organic reagent extraction.

-High quality: The extracted genomic DNA fragments are large, no RNA, no RNase, and extremely low ion content, which can meet the requirements of various experiments.

Kit application

It is suitable for the purification of the genomic DNA of the following samples: aquaculture pond water, pond water, tap water, lake water, river water, lotus pond water and other samples.

Workflow

Water DNA Isolation Kit

Storage and Shelf life

-This kit can be stored for 12 months under dry conditions at room temperature (15-25°C), if it needs to be stored for a longer period of time, it can be stored at 2-8°C.

Note: If stored at low temperature, the solution is prone to precipitation. Before use, be sure to put the solution in the kit at room temperature for a period of time. If necessary, preheat it in a 37°C water bath for 10 minutes to dissolve the precipitate, and mix it before use.

-Foregene Protease solution has a unique formula, which is active when stored at room temperature for a long time (3 months); when stored at 4℃, its activity and stability will be better, so it is recommended to store it at 4℃, remember not to store it at -20℃.

-Dry powder Lysozyme is stored at -20°C; the prepared Lysozyme solution is divided into small portions and stored at -20°C.


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