extract total RNA
Our firm sticks towards the basic principle of “Quality could be the life of your organization, and name may be the soul of it” for extract total RNA, Thanks for taking your worthwhile time to go to us and stay up for have a nice cooperation along with you.
Our firm sticks towards the basic principle of “Quality could be the life of your organization, and name may be the soul of it” for extract total RNA, Our company promises: reasonable prices, short production time and satisfactory after-sales service, we also welcome you to visit our factory at any time you want. Wish we’ve got a pleasant and long terms business together!!!
Specifications
50 Preps, 200 Preps
Kit components
Buffer RL1 , Buffer RL2 |
Buffer RW1, Buffer RW2 |
RNase-Free ddH2O, DNA-Cleaning Column |
RNA-Only Column |
Features&advantages
■ No need to worry about RNA degradation; the whole system is RNase-Free
■ Effectively remove DNA-using DNA-Cleaning Column
■ Remove DNA without adding DNase
■ Simple-all operations are completed at room temperature
■ Fast -operation can be completed in 30 minutes
■ Safe-no organic reagent required
■ High purity -OD260/280≈1.8-2.1
Kit application
It is suitable for the extraction and purification of total RNA from a variety of fresh or frozen animal tissues or cultured cells.
Product parameters
■ Downstream applications: first-strand cDNA synthesis, RT-PCR, molecular cloning, Northern Blot, etc.
■ Samples: animal tissues, cultured cells
■ Dosage: Tissues 10-20mg, Cells(2-5)×106
■ Maximum DNA binding capacity of purification column: 80 μg
■ Elution volume: 50-200 μl
Work flow
Diagram
Animal Total RNA Isolation Kit treated 20mg
Fresh mouse samples, take 5% purified total RNA 1% agar
Glycogel electrophoresis
1: Spleen 2: Kidney
3: Liver 4: Heart
Storage and shelf life
The kit can be stored for 24 months at room temperature (15–25 ℃) or 2–8 ℃ for longer time. Buffer RL1 can be stored at 4 ℃ for 1 month after adding β- mercaptoethanol (optional).
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