Foreasy RNase Inhibitor
Specifications
Foreasy RNase Inhibitor is a new mouse-derived RNase inhibitor expressed in E. coli engineered bacteria using gene recombination technology. The inhibitor inhibits RNase activity by non-competitively combining with RNase 1:1, thereby protecting RNA integrity , and can effectively inhibit the RNase A, B, C activity , but not RNase T1, T2, H, etc.; It is reversible of the binding of RNase Inhibitor and RNase , and the complex can be dissociated by urea and sulfhydryl reagents, so that RNase renatures and the inhibitor is irreversibly inactivated.
This product has the same application as the enzyme derived from human placenta. Foreasy RNase Inhibitor has high thermostability. It maintains high activity and inhibits RNase In the reverse transcription system using highly thermostable reverse transcriptase (such as: Foreasy Reverse Transcriptase).
Kit components
Component |
IM-03011 |
IM-03012 |
IM-03013 |
Foreasy RNase Inhibitor (40 U/μL) |
50 KU (1.25 mL) |
200 KU (5 mL) |
2000 KU (50 mL) |
Features&advantages
-High thermostability.
-It maintains high activity and inhibits RNase In the reverse transcription system using highly thermostable reverse transcriptase (such as: Foreasy Reverse Transcriptase).
Kit application
Foreasy RNase Inhibitor can be used in all experiments with RNase contamination or interference to protect RNA from degradation; in experiments, Foreasy RNase Inhibitor will not interfere or affect the experiment itself. For instance:
Synthesis of the first strand of cDNA, RT-PCR (direct RT-qPCR and direct RT-PCR)
In vitro transcription/translation
Inhibition of RNase activity during RNA isolation and purification
RNase protection experiment
Storage and Shelf life
Store at -20±5℃ for 2 years or at -80℃ for long-term storage.