ForeSNP Genotyping Kit
Kit Description:
High accuracy: typing the positive control, the accuracy rate is over 98%.
Low cost: No need to synthesize a large number of expensive dual-labeled probes.
Optimized PCR system: good system stability and strong amplification specificity.
Anti-pollution PCR system: It can effectively eliminate the aerosol pollution caused by PCR products, without worrying about the interference of environmental pollution on the fluorescent signal of the negative control, and ensure the specificity of amplification and the accuracy of typing.
Specifications
Competitive Allele Specific PCR (Competitive Allele Specific PCR) technology is a new type of allele typing method. This method does not need to synthesize specific probes for each SNP and inDel, but only needs two pairs of unique universal probes to achieve accurate typing of genomic DNA samples. By analyzing the intensity and ratio of the final fluorescence signal, the genotype is automatically determined, and the clustering effect is visually displayed. This method has short detection time, low reagent cost, high detection accuracy, and can be used for molecular marker-assisted breeding, QTL positioning, genetic marker identification and other molecular biology experiments with large sample volume.
Specifications
5ml, 50ml, 50ml×10, 500ml×20
Kit components
| 2× GT EasyTM Mix |
| DNase-Free ddH2O |
| Instructions |
Features&advantages
■ High accuracy: typing the positive control, the accuracy rate is over 98%.
■ Low cost: No need to synthesize a large number of expensive dual-labeled probes.
■ Optimized PCR system: good system stability and strong amplification specificity.
■ Anti-pollution PCR system: It can effectively eliminate the aerosol pollution caused by PCR products, without worrying about the interference of environmental pollution on the fluorescent signal of the negative control, and ensure the specificity of amplification and the accuracy of typing.
Kit appliacation
Intended for use in genotyping testing of purified DNA samples.
Example
In this experiment, 200ng wheat genomic DNA was used as a template to detect the typing of the TaGS-D1 gene related to the grain weight of wheat under the Foresnp Genotyping Kit. The instrument model is BIO-RAD CFX connect; the number of samples is 21, the number of NTCs is 8, and each type of positive control is 1.
ForeSNP Genotyping Kit
