Mouse Tail SuperDirect PCR Kit Direct PCR Lysis Reagent(Mouse Tail)(for Genotyping)
Kit Description:
Directly use mouse tail lysate as a template for PCR amplification, without separate extraction of DNA, fast and high sensitivity.
◮No time-consuming and expensive DNA purification is required.
◮The sample demand is small, just take a single seed.
◮No special treatments such as grinding and crushing are required, and the operation is simple.
◮The optimized PCR system enables PCR to have higher specificity and stronger tolerance to PCR reaction inhibitors.
Specifications
This kit uses a unique lysis buffer system to quickly release genomic DNA from mouse tail and mouse ear samples for PCR reaction, so it is particularly suitable for large-scale genetic testing.
The process of releasing genomic DNA from the lysis buffer is completed within 10-30 minutes at room temperature (20-25°C). No heating is required, and other processes such as protein and RNA removal are not required. The released trace amounts of DNA can be used as Template for PCR reaction.
2×M-PCR EasyTM Mix has strong tolerance to PCR reaction inhibitors, and can use the lysate of the sample to be tested as a template for efficient and specific amplification. This reagent contains Foregene D-Taq DNAPolymerase, dNTPs, MgCl2, reaction buffer, PCR optimizer and stabilizer.
D-Taq DNA polymerase is a DNA polymerase specially developed by Foregene for direct PCR reactions. D-Taq DNA polymerase has strong tolerance to a variety of PCR reaction inhibitors, and can efficiently amplify trace amounts of DNA in various complex reaction systems, and the amplification speed can reach 2Kb/min, which is especially suitable for Direct PCR reaction.
Specifications
50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns
Kit components
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Part I |
Buffer NP |
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Foregene Protease Plus I |
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6× DNA Loading Buffer |
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Part II |
2× M PCR EasyTM Mix |
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Instructions |
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Features&advantages
■ No time-consuming and expensive DNA purification is required.
■ The sample demand is small, just take mouse tails (or mouse ears with a diameter of 2-5mm) above 2mm.
■ Easy to operate, it can be lysed without heating to inactivate the protease, and the lysed product can be directly subjected to PCR reaction.
■ Foregene Mouse Tail Genomic DNA Extraction Kit (DE-05211) can be used to extract genomic DNA from the processed rat tail.
■ Optimized PCR system makes PCR have higher specificity and stronger tolerance to PCR reaction inhibitors.
Kit parameters
Application: Mouse tail, mouse ears, muscle and other tissues.
DNA released from sample lysis: only used as a PCR template.
The kit can be used for the following purposes: identification of transgenosis, animal genotyping, etc.
Work flow
Storage and Shelf life
Part I of this kit should be stored at 2-8℃.
The reagent Buffer FP can be stored for 12 months under dry conditions; it can be stored at 2-8℃ for longer storage.
Reagent Foregene Protease has a unique formula. To ensure its activity and stability, please store it at 4℃ for 12 months.
Reagent 6×DNA Loading Buffer can be stored at 4℃ or -20℃ for a long time.
Part II of this kit should be stored at -20℃.
Reagent 2×PCR EasyTM Mix, if is used frequently, it can also be stored at 4℃ for short-term storage (use up within 10 days).
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