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  • What are the differences between PCR, RT-PCR, qPCR, and RT-qPCR

    What are the differences between PCR, RT-PCR, qPCR, and RT-qPCR

    PCR is a method used to amplify DNA from a small amount of DNA template. RT-PCR uses reverse transcription to produce a DNA template from an RNA source that can then be amplified. PCR and RT-PCR are typically endpoint reactions, while qPCR and RT-qPCR use the kinetics of the rate of product synt...
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  • The Forefront of Drug Discovery: Targeting RNA with Small Molecules

    The Forefront of Drug Discovery: Targeting RNA with Small Molecules

    Pfizer’s mRNA vaccine for COVID has reignited the passion for using ribonucleic acid (RNA) as a therapeutic target. However, targeting RNA with small molecules is extremely challenging. RNA only has four building blocks: adenine (A), cytosine (C), guanine (G), and uracil (U) which replaces thymin...
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  • What is nucleic acid drugs?

    What is nucleic acid drugs?

    “Nucleic acid drugs” utilize “nucleic acid,” which refer to substances such as DNA and RNA that control genetic information, as drugs. These allow targeting of molecules such as mRNA and miRNA that cannot be targeted with traditional low molecular weight drugs and antibody drugs, and there is a g...
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  • Some experience about siRNA in RT-PCR

    Some experience about siRNA in RT-PCR

    1. Basic knowledge (if you want to see the experimental part, please transfer directly to the second part) As a derivative reaction of conventional PCR, Real time PCR mainly monitors the change of the amount of amplification product in each cycle of the PCR amplification reaction in real time thr...
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  • RT-PCR experimental reaction system optimization method detailed summary

    RT-PCR experimental reaction system optimization method detailed summary

    一、Increase the sensitivity of the reaction system: 1. Isolate high quality RNA: Successful cDNA synthesis comes from high-quality RNA. High-quality RNA should at least be full-length and free of reverse transcriptase inhibitors such as EDTA or SDS. The quality of RNA determines the maximum a...
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  • Complete PCR primer design and PCR details

    Complete PCR primer design and PCR details

    Primer design basis (99% problems can be solved) 1. Primer length: The textbook requires 15-30bp, usually about 20bp. The actual condition is better to be 18-24bp to ensure the specificity, but the longer the better, too long primer will also reduce the specificity, and reduce the yield. 2. Prim...
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  • Precautions for qRT-PCR compiled by senior Doctor painstakingly

    Precautions for qRT-PCR compiled by senior Doctor painstakingly

    Everyone is talking about the principle of qRT-PCR experiment, primer design, result interpretation, etc., but I think I should share with you the experimental operation of qRT-PCR. It’s small, but it’s about results. Before doing qRT-PCR, we need to have a clear understanding of our ...
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  • Precautions for RT-qPCR

    Precautions for RT-qPCR

    RT-qPCR experiment includes RNA extraction and quality assessment, reverse transcription and qPCR three steps, each step has a lot of precautions, we will introduce in detail below. Ⅰ. RNA quality assessment In RT-qPCR experiment, after the completion of RNA extraction, ...
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  • A thorough introduction to real time PCR

    A thorough introduction to real time PCR

    1. Initial understanding At this stage, we need to understand some concepts and terminology, so as to avoid making mistakes in front of our seniors, such as: Q: What is the difference between RT-PCR, qPCR, Real-time PCR, and real-time RT-PCR? Answer: RT-PCR is reverse transcription PCR (reverse t...
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  • PCR, multiple PCR, In situ PCR, Reverse PCR, RT-PCR, qPCR(1)– PCR

    PCR, multiple PCR, In situ PCR, Reverse PCR, RT-PCR, qPCR(1)– PCR

    PCR, multiple PCR, In situ PCR, Reverse PCR, RT-PCR, qPCR(1)– PCR We will sort out the concepts, steps and details of various PCR Ⅰ. PCR Polymerase Chain Reaction, referred to as PCR, is a molecular biological technology that is used to enlarge specific DNA fragments. It can be regarded a...
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  • qRT-PCR experimental principle

    qRT-PCR experimental principle

    RT-qPCR is developed from ordinary PCR technology. It adds fluorescent chemicals (fluorescent dyes or fluorescent probes) to the traditional PCR reaction system, and detects the PCR annealing and extension process in real time according to their different luminescent mechanisms. Fluorescent signa...
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  • RT-PCR experimental reaction system optimization method detailed summary

    RT-PCR experimental reaction system optimization method detailed summary

    Ⅰ. Increase the sensitivity of the reaction system: 1. Separate high -quality RNA: Successful cDNA synthesis comes from high -quality RNA. High -quality RNA should ensure at least a total longer and does not contain inhibitors that do not contain recording enzymes, such as EDTA or SDS. The quali...
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