Nucleic acid extraction is an introductory skill for molecular biology experiments. Whether it can master and use this skill proficiently has also become a prerequisite for later experimenters to upgrade to experimental masters.
According to different research materials, experimenters are mainly divided into the following three types:
Animal major, Plant major, Microbiology major
Each type of experimenter will continue to copy copies to cultivate a set of exclusive skills for specific samples.
Today, the editor wants to bring some equipment and props that are not available through the dungeon to the experimenters. With them, you can quickly upgrade in all directions. It is not a dream to become an experimental god.
This issue is for plant professional experimenters (experimenters of other professions can also learn from it~).
I believe that every generation of experimenters will have heard of the classic plant nucleic acid extraction methods passed down from generation to generation from their predecessors. The three big names in the plant extraction industry.
Although the big guys are excellent, they still have some unavoidable shortcomings.
TRIzol method
Often used to extract
RNA
●Toxic and cumbersome
●Containing genome contamination
CTAB method
Often used to extract
genome DNA
●Time consuming
●More difficult to remove CTAB by washing than salt, a small amount of residue will have a huge impact on enzyme activity
SDS method
Often used to extract
plasmid
●Not suitable for plants with high polysaccharide content
●Protein contamination
In this modern society with double-speed update iterations, traditional methods of operation are no longer able to meet the needs of the experimenters, and equipment upgrades are imminent.
Foregene Nucleic Acid Extraction Kit has been tested N times by dozens of experimenters. It is simple to operate, does not use any toxic reagents, and does not add any enzymes. The nucleic acid obtained is of high purity, which is essential for the daily extraction operation of excellent experimenters.
Foregene RNA Isolation kit
Here, I will also reveal a hidden operation technique to the experimenters, which is to choose the appropriate extraction method according to the plant type (the method to determine the plant type is at the end of the article!), because some plant polysaccharides and polyphenols are high in content, and traditional methods are lethal. Not enough to control.
Well, it's like this...
The TRIzol method directly kneeled under samples with high levels of polysaccharides and polyphenols such as banana leaves, ginkgo leaves and cotton leaves.
For this purpose, Foregene has developed two effective tools for experimenters. The plant RNA extraction kit with low polysaccharide and polyphenol content and high polysaccharide and polyphenol content can kill the enemies in a targeted manner, so that the experimenters are no longer afraid of different plants. Just change the equipment directly!
In addition to the need to upgrade the extraction method, the sample pre-processing operations also need to be upgraded.
Because of the special structure of plant samples, the roots and tougher stems of some plants are more difficult to grind and crush evenly.
In the grinding industry, about 80% of people use liquid nitrogen for grinding, while less than 20% use electric grinding and grinding bead oscillation.
Although liquid nitrogen grinding is cheap and easy to use, it is a nightmare for experimenters with a large sample demand to grind each time and effort! If the mortar is not cleaned, it will cause cross-contamination between samples.
The effect of electric grinding varies from person to person, and the operating habits of the experimenters will have a greater impact on the integrity of the nucleic acid, and low-temperature operation is required if necessary.
so, grinding bead oscillation can be regarded as the current simple and effective grinding equipment.
The common oscillation modes include vertical oscillation, horizontal reciprocating oscillation and three-dimensional (horizontal oscillation). However, Foregene's tissue crusher breaks through the existing model and adopts a new 4D oscillation method to make the grinding more uniform.
Meet various sample requirements, easy to operate
Unique 4D crushing technology, the crushing efficiency is increased by 20%
The sample is completely enclosed, no cross-contamination
Quiet, ≤56 decibels
Compare the results of the experiment to a wave~
Test sample: 50mg wheat leaves (low content of polysaccharides and polyphenols)
Extract nucleic acid type: RNA
Spot amount: 5μl
The extraction kit used is Foregene Plant Total RNA Extraction Kit (RE-05011)
Comparing the data and the electrophoresis graph, it can be seen that the concentration and purity of the RNA extracted after the machine grinding is higher than that of the liquid nitrogen grinding. At the same time, because the machine grinding can break the sample more completely, the small RNA can be better extracted and the integrity of the RNA can be maintained.
Test sample: 50mg ginkgo leaves (high polysaccharide and polyphenol content)
Extract nucleic acid type: RNA
Spot amount: 5μl
Using Foregene Polysaccharide Polyphenol Plant Total RNA Extraction Kit (RE-05021)
Take a look at this group of samples with high content of polysaccharides and polyphenols. The effect is even more obvious. With the same purity, the concentration of machine-grinding RNA is nearly 5 times that of liquid nitrogen grinding, and small RNA is still faintly visible~
If you feel that none of the above equipment is fast enough, then the editor has to come up with the ultimate equipment!
Plant Direct PCR series
The unique feature of this equipment is that the plant leaves or seeds do not need to be ground and broken, and are directly thrown into the EP tube for lysis, and the resulting lysis mixture can be used as a template for PCR reaction. It is as simple as one step faster! At the same time, the direct PCR series of Foregene Plants are equipped with the UNG anti-pollution system, so that the experimenters no longer worry about aerosol pollution in the laboratory and can easily take the experiment.
Judgment tips:
How to judge whether it is a polysaccharide polyphenol plant
Take about 20mg tissue block and put it into a clean 1.5ml centrifuge tube, add 200ul 200mM NaOH solution, place it at 95℃ for 10min, observe the color change after lysis
1. If the color of the lysate is green or light yellow, it means that the content of tissue polyphenols is low, and it is recommended to use the Foregene Plant series
2. If the lysate is brownish-yellow or brownish-red, it means that the tissue polyphenol content is high, and it is recommended to use Foregene Polysaccharide Polyphenol Plant Plus Series
Post time: Aug-20-2021
