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The birth of PCR

PCR(Polymerase Chain Reaction)

It has been more than 30 years since the invention of polymerase chain reaction. For more than 30 years, after numerous scholars around the world continue to supplement and improve, PCR technology has become the most widely and frequently used and most important basic research method in the entire Life Sciences field.

The TouchDown PCR, Real-Time PCR, Multi PCR, etc. developed on the basis of the wide application of traditional PCR technology, as well as the newly emerged Digital PCR (digital PCR), have greatly enriched the research methods of the majority of scientific researchers and greatly accelerated the development process of modern Life Sciences, especially molecular biology, has made great contributions to the study of life and nature of mankind as a whole.

PCR-principle
Polymerase-Chain-Reaction-PCR

Defects of traditional PCR technology

Complex nucleic acid separation and extraction:

★ Traditional PCR technology: required

★ PCR derived technology: required

★ DNA and RNA samples: large differences, difficult operation requirements

★ Body hazards: toxic reagents harm the body

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Traditional PCR technology and derivative technology have a prerequisite-nucleic acid separation and purification

Any biological sample needs to go through a series of complicated and tedious sample processing to obtain nucleic acid samples that meet the requirements of PCR technology.

The separation and extraction of DNA and RNA has always been a basic task that relevant scientific researchers need to repeat every day.

Due to the huge differences between samples, the separation and extraction processes of DNA and RNA are also very different. This work requires a high level of technical proficiency for operators. Traditional separation and extraction techniques require long-term contact with some highly toxic chemical reagents. It will cause irreversible damage to the operator’s body, and even cause direct damage during the experiment.

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At the same time, for those who have a large number of samples to study, separation and extraction of nucleic acids is a labor-intensive task.

Nucleic acid isolation and extraction kits on the market are now mature and there are many brands, but they are roughly the same. Whether it is a silica gel membrane column centrifugal kit or a magnetic bead method kit, it takes a lot of time and is costly. In addition to the cost of the kit, there are also special requirements for laboratory equipment. The automated workstation used in the magnetic bead method is a very typical large-scale high-value equipment, which is a huge expense for the laboratory.

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In summary

Before conducting PCR experiments, the pretreatment of samples is an inevitable and always headache for researchers. How to solve this problem and whether PCR experiments can be performed without the separation and extraction of nucleic acids has always been the thinking of the majority of scientific researchers and clinical laboratory personnel.

Foregene’s Solution

After years of painstaking research on Direct PCR technology and related kits, Forgene successfully broke through many bottlenecks and successfully achieved direct PCR for many types of different samples with strong resistance and adaptability, allowing researchers to get rid of cumbersome and dangerous separation and extraction of nucleic acids. This will greatly reduce everyone’s labor intensity, speed up the experiment process, and save scientific research and testing costs.

Forgene’s understanding and knowledge of DirectPCR

First, DirectPCR technology is a direct PCR technology for various biological sample tissues. Under this technical condition, there is no need to separate and extract nucleic acids, and the tissue sample is directly used as the object, and the target gene primers are added for PCR reaction.

Second, DirectPCR technology is not only a traditional DNA template amplification technology, but also includes RNA template reverse transcription PCR.

Third, DirectPCR technology not only directly performs routine qualitative PCR reactions on tissue samples, but also includes Real-Time qPCR reactions, which requires the reaction system to have a strong ability to resist background fluorescence interference and to antagonize endogenous fluorescence quenchers.

Fourth, the tissue samples targeted by the DirectPCR technology only require the release of nucleic acid templates and do not remove proteins, polysaccharides, salt ions, etc. that interfere with the PCR reaction. This requires the nucleic acid polymerase and PCR Mix in the reaction system to have excellent anti-reversibility and adaptability,and can ensure enzyme activity and replication accuracy under complex conditions.

 Fifth, the tissue samples targeted by the DirectPCR technology have not been subjected to any nucleic acid enrichment treatment, and the template quantity is very small, which requires extremely high sensitivity and amplification efficiency of the reaction system.

Conclusion

DirectPCR technology is one of the most important technological developments and innovations in the past 30 years since the birth of PCR technology. Forgene has and will continue to be a pioneer and innovator of this technology.

The application prospect of DirectPCR technology is very broad. The continuous improvement and promotion of this technology will surely bring subversive changes to scientific research and inspection work. This is a PCR technology revolution.


Post time: Feb-21-2017