What is DirectPCR?
DirectPCR technology means that without separating and purifying nucleic acid molecules (including DNA and RNA) of various tissue samples, only the tissue and cell structure is destroyed by the lysis of protease, the nucleic acid is released into the lysis solution, and the lysis solution is directly added. The PCR reaction system is a technology for amplification of the target gene.
Looking for change
Separation and extraction of nucleic acids issue
Since the invention of PCR technology 30 years ago, researchers have been troubled by the separation and extraction of nucleic acids. Using tissue samples to directly perform PCR reactions is the dream of many researchers. But this dream has not been realized for 30 years. The reason is that the disintegrated tissue will release a lot of inhibitory ingredients.
These inhibitory components will have a strong inhibitory effect on the PCR reaction, some will cause the primer and the template to be unable to bind, some have a strong protein denaturation function, resulting in the inactivation of nucleic acid polymerase, and some directly prevent the primer and the template from binding . These are all factors that cause the PCR reaction to not proceed smoothly.
Traditionally, people occasionally use PCR enhancers to enhance the sensitivity, specificity and amplification efficiency of PCR reactions. However, due to the different PCR inhibitors contained in nucleic acid templates from different sources, long-term use of a large number of enhancers is obviously not worth the loss, the cost is high, and the operation is cumbersome.
Foregene DirectPCR
The world's leading breakthrough --Two Techniques
Foregene has made breakthroughs in this technology field on a global scale. Foregene DirectPCR technology has two technical points, which enables Foregene to become the pioneer and leader of DirectPCR technology.
First, the patented nucleic acid polymerase modification method. Forgene has a patented nuclease modification method, which is targeted at the binding domain to make the nuclease and the template stronger, with higher activity, higher amplification efficiency and thermal stability.
Second, the PCR Mix formula optimized for different species, unique reaction enhancers, optimizers and stabilizers, greatly enhance the polymerase's resistance to PCR inhibitors and ensure amplification efficiency.
It is precisely because of the above-mentioned two important technological breakthroughs that Foregene has realized the real DirectPCR on a global scale. Whether it is common animal tissues, body fluids, plant tissues, leaves or root tips, or even plant seeds, users can easily achieve direct PCR amplification without any mechanical breakage or tedious nucleic acid purification process.
For the DirectPCR kits of the plant series and animal series that have been launched, Foregene can proudly say that we have the world's leading performance indicators. In the future, Foregene will continue to launch a series of products that still have world-leading performance indicators (or even unique).
For more info, enter :
http://www.foregene.com/ http://www.foreivd.com/
Post time: Feb-27-2017
