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Published by: CAS Key Laboratory of Pathogen Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences

IF: 69.504

Assisted Product: Cell Total RNA Isolation Kit

Original Link: https://www.nature.com/articles/s41586-022-05271-2

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Infections with filoviruses such as Ebola virus (EBOV) and Marburg virus can cause severe clinical symptoms, including haemorrhagic fever and multiorgan failure4. Here we determined the structure of Ebola virus L protein in complex with tetrameric VP35 using cryo-electron microscopy (state 1). Structural analysis revealed that Ebola virus L possesses a filovirus-specific insertion element that is essential for RNA synthesis, and that VP35 interacts extensively with the N-terminal region of L by three protomers of the VP35 tetramer.

In this paper, total RNA was extracted using Foregene Cell Total RNA Isolation Kit and the concentration of RNA was measured by Nanodrop at 260 nm, and then perform RT/qPCR detection.

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■ The whole process is operated at room temperature (15-25℃), without ice bath and low temperature centrifugation.

■ The whole kit is RNase-Free, no need to worry about RNA degradation.

■ DNA-Cleaning Column specifically binds DNA, so that the kit can remove genomic DNA contamination without adding additional DNase.

■ High RNA yield: RNA-only Column and unique formula can efficiently purify RNA.

■ Fast speed: easy to operate and can be completed in 11 minutes.

■ Safety: No organic reagent is required.

■ High quality: The purified RNA is of high purity, free of protein and other impurities, and can meet various subsequent experiments.


Post time: Nov-04-2022