lncRNA features:

1. lncRNAs are usually longer, with dynamic expression and different splicing methods during differentiation;

2. Compared with coding genes, lncRNA is usually lower;

3. Most lncRNAs have obvious temporal and spatial expression specificity in the process of tissue differentiation and development;

4. There are characteristic expressions in tumors and other diseases;

5. The subcellular locations of lncRNA are diverse;

6. The sequence is low in conservation, but the function has a certain degree of conservation and so on.

LncRNA reverse transcription solution:

We know that lncRNA has a high-level structure in cells. Such RNA often has complex structures such as stem loops or hairpins. If the secondary structure of lncRNA cannot be solved effectively, reverse transcription is prone to be unsuccessful or unsuccessful when doing RT-qPCR. The problem of low efficiency.

Generally, there are three key points for us to obtain high-quality cDNA templates:

RNA template quality, reverse transcription primers and reverse transcriptase, and reverse transcriptase is the core key factor. The RNA purified by commonly used RNA extraction reagents often contains residues of alcohol and guanidine salts, which have strong inhibitory effects on most reverse transcriptases; if the optimum temperature for the reverse transcriptase reaction is low, it will not be conducive to RNA. The opening of the hierarchical structure.

At present, the suitable temperature for reverse transcriptase used in most laboratories is generally 42°C, and the maximum temperature can reach about 50°C. In fact, the opening of the higher-order RNA structure requires a higher temperature.

The temperature for synthesizing the first-strand cDNA of Fortune Biotech 2×RT EasyTM Mix is as high as 50℃-65℃, which is conducive to completely unraveling the high-level structure of lncRNA, and the operation time is short.

In addition, the product also has strong anti-interference ability for RNA with residual alcohol and guanidine salt, which can fully meet the needs of laboratories to achieve high reverse transcription efficiency.

First Strand cDNA Synthesis Kit

Item No.: RT-01011 (25 times)

Features:

1. Thermal stability: 65℃ still has good reverse transcription activity, which helps to open the complex secondary structure

2. Tolerance: cDNA synthesis can still be successfully performed on RNA templates with poor purity (ethanol and guanidine salt residues)

3. Fast: simple operation, the first strand cDNA synthesis can be completed within 25-35 minutes

Downstream applications: PCR, qPCR, RACE, chip labeling, library construction, etc.

In addition, FJ Biotech provides a full set of RT-PCR solutions to meet the needs of different types of customers, including the following kits:

First Strand cDNA Synthesis Kit (For qPCR)

Item No.: RT-01021 (50 times)

Features:

1. Convenience: Reverse transcription primers have been added to make the operation easier

2. Thermal stability: 65℃ still has good reverse transcription activity, which helps to open the complex secondary structure

3. Tolerance: cDNA synthesis can still be successfully performed on RNA templates with poor purity

4. Sensitivity: suitable for low-concentration RNA samples

Downstream application: Specially provided for subsequent qPCR

One-step RT-PCR kit

Item No.: RT-02011 (100 times)

Features:

1. Thermal stability: 65℃ still has good reverse transcription activity, which helps to open the complex secondary structure

2. Tolerance: cDNA synthesis can still be successfully performed on RNA templates with poor purity

3. Specificity: The equipped PCR MIX effectively improves the amplification efficiency and specificity of the reaction

Downstream applications: cDNA cloning, genetic testing and other molecular experiments

Two-step RT-PCR kit

Item No.: RT-02021 (100 times)

Features:

1. Simple and fast: one-tube reaction

2. Thermal stability: 65℃ still has good reverse transcription activity, which helps to open the complex secondary structure

3. Specificity: The added Foregene Taq effectively improves the amplification efficiency and specificity of the reaction

Downstream applications: molecular experiments such as genetic testing

One-step RT-qPCR kit-SYBR Green I

Item No.: RT-02111 (100 times)

Features:

1. Simple: one-tube reaction

2. High sensitivity: can detect low copy templates

3. Compatibility: suitable for most fluorescence quantitative instruments

Downstream applications: detection of trace RNA samples such as cells and viruses

One-step RT-qPCR kit-Taqman

Item No.: RT-02131 (100 times)

Features:

1. Fast: detect the target gene

2. High sensitivity: can detect low copy templates

3. Compatibility: suitable for most fluorescence quantitative instruments

Downstream applications: detection of trace RNA samples such as cells and viruses
Forge Bioreverse Transcription Kit can help you better solve the problem of reverse transcription. At the same time, it cooperates with Forge Bio RNA Extraction Kit (which can effectively remove DNA residues) to provide you with a complete RNA experimental solution.