Industrial News
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Pay attention to the following details to double the success rate of your RT-qPCR experiment!
RT-qPCR is the basic experiment of molecular biology, and everyone must be familiar with it. It mainly includes three steps: RNA extraction, reverse transcription into cDNA, and real-time fluorescent quantitative PCR. It doesn’t help, what is going on? It is likely that there is a problem w...Read more -
RNA extraction, the 260/230 is particularly low, and the ct value of pcr run is very high. How to solve it?
The low ratio of A260/A230 is usually caused by impurities with the maximum absorption wavelength at 230nm. Let’s see what these impurities include: Common pollutants Absorption wavelength Ratio effect Protein ~230nm and 280nm Simultaneous reduction of A 260 /A 280 and A 260 /A ...Read more -
Fluorescent quantitative PCR (qPCR) – primer design
In qPCR experiments, primer design is also a very important link. Whether the primers are suitable or not is closely related to whether the amplification efficiency reaches the standard, whether the amplified products are specific, and whether the experimental results are available. So how to mak...Read more -
Common additives in PCR reactions and their functions
I believe that everyone will always encounter such or such problems when doing PCR reactions, but most of them can be classified into two main problems: Too little amplification of the gene template (amplification); Too much non-target gene amplification. Using additives is one of the common stra...Read more -
A summary of 45 gene therapy drugs that have been marketed around the world
Genes are the basic genetic units that control traits. Except for the genes of some viruses, which are composed of RNA, the genes of most organisms are composed of DNA . Most diseases of organisms are caused by the interaction between genes and the environment. Gene therapy can essentially cure o...Read more -
In the era of RNA therapy, who can become the “new darling” of the industry | Annual inventory
Source: WuXi AppTec Recent years, the field of RNA therapy has shown an explosive trend- in the past 5 years alone, 11 RNA therapies have been approved by the FDA, and this number even exceeds the sum of previously approved RNA therapies! Compared with traditional therapies, RNA therapy ca...Read more -
Ordinary PCR, fluorescent PCR, digital PCR; analysis of advantages and disadvantages!
Several revolutionary inventions in the history of detection technology in my mind are immunolabeling technology based on the principle of antigen-antibody specific binding, PCR technology and sequencing technology. Today we will talk about PCR technology. According to t...Read more -
Summary|As of August 2022, 41 gene therapy drugs have been launched worldwide.
Recently, three gene therapy drugs have been approved for marketing, namely: (1) On July 21, 2022, PTC Therapeutics, Inc. (NASDAQ: PTCT) announced that its AAV gene therapy Upstaza™ was approved by the European Commission It is the first marketed gene therapy directly in...Read more -
The Past and Present Of CACLP
From October 26th to 28th, 2022, a new feast of experimental medicine and in vitro diagnosis – The 19th China China Association of Clinical Laboratory Practice Expo The 2nd China IVD Supply Chain Expo (CISCE) was successfully held at Nanchang Greenland International Expo Center. FOREGENE Co...Read more -
PCR machine|Do you really understand?
PCR machine|Do you really understand? Nobel Prize-winning PCR technology In 1993, American scientist Mulis received the Nobel Prize in Chemistry, and his achievement was the invention of PCR technology. The magic of PCR technology is in the following characteristics: Firstly, the amount of DNA to...Read more -
Latest Nature Thesis: Analyze the structure and cutting DNA mechanism of the new gene editing tool ISRB
In the past ten years, the gene editing technology based on CRISPR has developed rapidly, and has been successfully applied to the treatment of genetic diseases and cancer in human clinical trials. At the same time, scientists around the world are constantly tap new new tools with gene editing po...Read more -
Gene therapy, completely “wake up” the ear
Hearing loss (HL) is the most common sensory disability disease in humans. In developed countries, about 80% of prelingual deafness cases in children are caused by genetic factors. The most common are single-gene defects (as shown in Fig. 1), 124 gene mutations have been found to be associated wi...Read more
