Blood DNA Mini Kit
Kit Description:
-No RNase contamination: The DNA-Only Column provided by the kit makes it possible to remove RNA from genomic DNA without adding RNase during the experiment, avoiding the laboratory from being contaminated by exogenous RNase.
-Fast speed: Foregene Protease Plus has higher activity than similar proteases, and digests tissue samples faster; the operation is simple, and the genomic DNA extraction operation can be completed within 40 minutes.
-Convenience: The centrifugation is performed at room temperature, 4°C centrifugation or ethanol precipitation of DNA is not required.
-Safety: No organic reagent extraction is used.
-High quality: The extracted genomic DNA fragments are large, no RNA, no RNase, and extremely low ion content, which can meet the requirements of various experiments.
Specifications
50 Preps, for genomic DNA purification from whole blood using <1ml blood
Buffer DC: Lyses red blood cells in the blood.
Buffer BL1: Provide a sample enzymatic digestion environment.
Foregene Protease Plus: Enzymatically digest blood samples in the environment of Buffer BL1.
Buffer BL2: Inactivate Foregene Protease Plus and provide a DNA loading environment.
Buffer PW: Remove impurities such as protein and RNA in DNA.
Buffer WB1: Remove residual salt ions in DNA.
Buffer EB: Elute the DNA on the purification column membrane.
DNA-Only Column: Specifically adsorbs genomic DNA in the lysate.
Kit components
|
Buffer BL1 |
| Buffer BL2* |
| Buffer DC |
| Buffer PW* |
| Buffer WB1 |
| Buffer EB |
| Foregene Protease Plus |
| DNA-Only Column |
|
Instructions |
Features&advantages
-No RNase contamination: The DNA-Only Column provided by the kit makes it possible to remove RNA from genomic DNA without adding RNase during the experiment, avoiding the laboratory from being contaminated by exogenous RNase.
-Fast speed: Foregene Protease Plus has higher activity than similar proteases, and digests tissue samples faster; the operation is simple, and the genomic DNA extraction operation can be completed within 40 minutes.
-Convenience: The centrifugation is performed at room temperature, 4°C centrifugation or ethanol precipitation of DNA is not required.
-Safety: No organic reagent extraction is used.
-High quality: The extracted genomic DNA fragments are large, no RNA, no RNase, and extremely low ion content, which can meet the requirements of various experiments.
Kit application
It is suitable for extraction and purification of genomic DNA from fresh or frozen anticoagulated whole blood.
Workflow
Storage and Shelf life
This kit can be stored for 12 months under dry conditions at room temperature (15-25°C); if it needs to be stored for a longer period of time, it can be stored at 2-8°C.
Note: If stored at low temperature, the solution is prone to precipitation. Before use, be sure to place the solution in the kit at room temperature for a period of time. If necessary, preheat it in a 37°C water bath for 10 minutes to dissolve the precipitate, and mix it before use.
The Foregene Protease Plus solution has a unique formula, which is active when stored at room temperature for a long time (3 months); it will be more active and stable when stored at 4°C, so it is recommended to store it at 4°C, remember not to keep it at -20°C.