Plant leaf Direct PCR kit (without Sampling Tools) Protocol Direct PCR from Plant Material
Descriptions
This product uses a unique lysis buffer system to lyse plant leaves. The lysate can be used as a template without purification. After adding the primers, the PCR Mix of this kit can be used for amplification.
Specifications
0×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns
Kit components
Part I |
Buffer P1 |
Buffer P2 |
|
6×DNA Loading Buffer |
|
Part II |
2× Leaf PCR EasyTM Mix |
Features&advantages
■ No time-consuming and expensive DNA purification
■ Less material
■ Simple-the sample can be subjected to PCR reaction or lysis reaction without cutting or grinding
■ 2 × Mix, reducing sample loading errors and reaction system preparation time
■ Fast-template preparation can be completed in 10 minutes, PCR reaction can be completed in at least 50 minutes
■ High throughput-lysis reaction can be completed in 96-well PCR plate
Kit parameters
Application: genetically modified identification, genotyping, etc.
Sample: plant leaf
Dosage: Diameter 2-3mm (direct method), diameter 5-7mm (lysis method)
Detection range: target fragment ≤1kb