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Plant leaf Direct PCR kit (without Sampling Tools) Protocol Direct PCR from Plant Material

Kit Description:

Directly use leaf or leaf lysate as a template for PCR amplification, without separate extraction of DNA, fast and high sensitivity.

No time-consuming and expensive DNA purification(Time, labor and cost saved!)

Less material

Simple—the sample can be subjected to PCR reaction or lysis reaction without cutting or grinding

2 X Mix—reducing sample loading errors and reaction system preparation time

Fast—template preparation can be completed in 10 minutes, PCR reaction can be completed in 50 minutes

High throughput—lysis reaction can be completed in 96-well PCR plate

foregene strength


Product Detail

Product Tags

Descriptions

This product uses a unique lysis buffer system to lyse plant leaves. The lysate can be used as a template without purification. After adding the primers, the PCR Mix of this kit can be used for amplification.

Specifications

0×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Kit components

Part I

Buffer P1

Buffer P2

6×DNA Loading Buffer

Part II

2× Leaf PCR EasyTM Mix

Features&advantages

■ No time-consuming and expensive DNA purification
■ Less material
■ Simple-the sample can be subjected to PCR reaction or lysis reaction without cutting or grinding
■ 2 × Mix, reducing sample loading errors and reaction system preparation time
■ Fast-template preparation can be completed in 10 minutes, PCR reaction can be completed in at least 50 minutes
■ High throughput-lysis reaction can be completed in 96-well PCR plate

Kit parameters

Application: genetically modified identification, genotyping, etc.
Sample: plant leaf
Dosage: Diameter 2-3mm (direct method), diameter 5-7mm (lysis method)
Detection range: target fragment ≤1kb

Work flow

Plant Leaf Direct PCR Kit04

Electropherogram

Plant Leaf Direct PCR Kit05

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