Plant leaf Direct PCR kit-UNG
Based on Plant Leaf Direct PCR kit, dUTP is used instead of dTTP, and the UNG enzyme that can degrade the template containing dUTP is added. In this way, the specificity and accuracy of amplification are ensured, and the problem of PCR product contamination that may occur during large-scale genetic testing is prevented.
50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns
6×DNA Loading Buffer
2× Leaf PCR EasyTM Mix(UNG)
■ No time-consuming and expensive DNA purification
■ Less material
■ Simple-the sample can be subjected to PCR reaction or lysis reaction without cutting or grinding
■ 2 × Mix, reducing sample loading errors and reaction system preparation time
■ Fast-template preparation can be completed in 10 minutes, PCR reaction can be completed in at least 50 minutes
■ High throughput-lysis reaction can be completed in 96-well PCR plate
Application: genetically modified identification, genotyping, etc.
Sample: plant leaf
Dosage: Diameter 2-3mm (direct method), diameter 5-7mm (lysis method)
Detection range: target fragment ≤1kb
Two weeks of PCR Mix detection results under the condition of PCR amplification product contamination.