Plant leaf Direct PCR kit-UNG

Kit Description:

No time-consuming and expensive DNA purification

Less material

Simple—the sample can be subjected to PCR reaction or lysis reaction without cutting or grinding

2X Mix—reducing sample loading errors and reaction system preparation time

Fast—template preparation can be completed in 10 minutes, PCR reaction can be completed in 50 minutes

High throughput—lysis reaction can be completed in 96-well PCR plate

foregene strength


Product Detail

Product Tags

Descriptions

Based on Plant Leaf Direct PCR kit, dUTP is used instead of dTTP, and the UNG enzyme that can degrade the template containing dUTP is added. In this way, the specificity and accuracy of amplification are ensured, and the problem of PCR product contamination that may occur during large-scale genetic testing is prevented.

Specifications

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Kit components

Part I

Buffer P1

Buffer P2

6×DNA Loading Buffer

Part II

2× Leaf PCR EasyTM Mix(UNG)

Features&advantages

■ No time-consuming and expensive DNA purification
■ Less material
■ Simple-the sample can be subjected to PCR reaction or lysis reaction without cutting or grinding
■ 2 × Mix, reducing sample loading errors and reaction system preparation time
■ Fast-template preparation can be completed in 10 minutes, PCR reaction can be completed in at least 50 minutes
■ High throughput-lysis reaction can be completed in 96-well PCR plate

Kit parameters

Application: genetically modified identification, genotyping, etc.
Sample: plant leaf
Dosage: Diameter 2-3mm (direct method), diameter 5-7mm (lysis method)
Detection range: target fragment ≤1kb

Work flow

Plant Leaf Direct PCR Kit-UNG4

Electropherogram

Plant Leaf Direct PCR Kit-UNG5

Two weeks of PCR Mix detection results under the condition of PCR amplification product contamination.


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