Plant leaf Direct PCR Plus kit (without Sampling Tools) Protocol Direct PCR from Plant Material
This product uses a unique lysis buffer system to lyse plant leaves. The lysate can be used as a template without purification. After adding the primers, the PCR Mix of this kit can be used for amplification.
50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns
|6×DNA Loading Buffer
|2× Leaf PCR EasyTM Mix
■ No time-consuming and expensive DNA purification
■ Less material
■ Simple-the sample can be subjected to PCR reaction or lysis reaction without cutting or grinding
■ 2 × Mix, reducing sample loading errors and reaction system preparation time
■ Fast-template preparation can be completed in 10 minutes, PCR reaction can be completed in at least 50 minutes
■ High throughput-lysis reaction can be completed in 96-well PCR plate
Application: Identification of transgenic plants, genotyping, etc.
Sample: plant leaves rich in polysaccharide or polyphenol
Sample size: Diameter 2-3mm (direct method), diameter 5-7mm (lysis method)
Detection range: PCR product ≤1kb