Plant Seed Direct PCR Kit I/II (without Sampling Tools) Protocol Plant Direct PCR Kits
Descriptions
This product uses a unique lysis buffer system to lyse plant seeds. The lysate can be used as a template without purification. After adding the primers, the PCR Mix of this kit can be used for amplification.
Specifications
50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns
Kit components
Plant Seed Direct PCR Kit I |
|
Part I |
Buffer SP1 |
Buffer SP2 |
|
6×DNA Loading Buffer |
|
Part II |
2×Seed PCR EasyTM Mix |
Plant Seed Direct PCR Kit II |
|
Part I |
Buffer SP1 |
Buffer SP2 |
|
6×DNA Loading Buffer |
|
Part II |
2×Seed PCR EasyTM Mix |
Features&advantages
■ No time-consuming and expensive DNA purification
■ Less material
■ Simple—the sample can be subjected to PCR reaction or lysis reaction without cutting or grinding
■ 2X Mix—reducing sample loading errors and reaction system preparation time
■ Fast—template preparation can be completed in 10 minutes, PCR reaction can be completed in 50 minutes
■ High throughput—lysis reaction can be completed in 96-well PCR plate.
Kit parameters
Application: Identification of transgenic plants, genotyping, etc.
Sample: Plant seeds or tissues with low polysaccharide and polyphenol content.
Sample size: Single whole seed, 1-100mg plant tissue
Detection range: PCR product ≤1kb