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Plant Seed Direct PCR Kit I/II (without Sampling Tools) Protocol Plant Direct PCR Kits

Kit Description:

No time-consuming and expensive DNA purification(Time, labor and cost saved!)

Less material

Simple—the sample can be subjected to PCR reaction or lysis reaction without cutting or grinding

2 X Mix—reducing sample loading errors and reaction system preparation time

Fast—template preparation can be completed in 10 minutes, PCR reaction can be completed in 50 minutes

High throughput—lysis reaction can be completed in 96-well PCR plate

foregene strength


Product Detail

Product Tags

Descriptions

This product uses a unique lysis buffer system to lyse plant seeds. The lysate can be used as a template without purification. After adding the primers, the PCR Mix of this kit can be used for amplification.

Specifications

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Kit components

Plant Seed Direct PCR Kit I

Part I

Buffer SP1

Buffer SP2

6×DNA Loading Buffer

Part II

2×Seed PCR EasyTM Mix


Plant Seed Direct PCR Kit  II

Part I

Buffer SP1

Buffer SP2

6×DNA Loading Buffer

Part II

2×Seed PCR EasyTM Mix

Features&advantages

■ No time-consuming and expensive DNA purification

■ Less material

■ Simplethe sample can be subjected to PCR reaction or lysis reaction without cutting or grinding

■ 2X Mixreducing sample loading errors and reaction system preparation time

■ Fasttemplate preparation can be completed in 10 minutes, PCR reaction can be completed in 50 minutes

■ High throughputlysis reaction can be completed in 96-well PCR plate.

Kit parameters

Application: Identification of transgenic plants, genotyping, etc.

Sample: Plant seeds or tissues with low polysaccharide and polyphenol content.

Sample size: Single whole seed, 1-100mg plant tissue

Detection range: PCR product ≤1kb

Work flow

plant seed direct PCR

Electropherogram

plant seed direct PCR-DIAGRAM

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