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Plant Seed(Polysaccharide Polyphenol rich,large and medium-sized) Direct PCR Plus Kit II-UNG(without Sampling Tools)

Plant Seed(Polysaccharide Polyphenol rich,large and medium-sized) Direct PCR Plus Kit II-UNG(without Sampling Tools) Featured Image
  • Plant Seed(Polysaccharide Polyphenol rich,large and medium-sized) Direct PCR Plus Kit II-UNG(without Sampling Tools)
  • Plant Seed(Polysaccharide Polyphenol rich,large and medium-sized) Direct PCR Plus Kit II-UNG(without Sampling Tools)
  • Plant Seed(Polysaccharide Polyphenol rich,large and medium-sized) Direct PCR Plus Kit II-UNG(without Sampling Tools)
  • Plant Seed(Polysaccharide Polyphenol rich,large and medium-sized) Direct PCR Plus Kit II-UNG(without Sampling Tools)

Kit Description:

Directly use polysaccharide polyphenol seed lysate as a template for PCR amplification, without separate extraction of DNA, fast and high sensitivity. The UNG anti-pollution system has been added to prevent false positives.

◮No time-consuming and expensive DNA purification is required.

◮The sample demand is small, just take a single seed.

◮No special treatments such as grinding and crushing are required, and the operation is simple.

◮The optimized PCR system enables PCR to have higher specificity and stronger tolerance to PCR reaction inhibitors.

foregene strength


Product Detail

Product Tags

Descriptions

This product uses a unique lysis reaction system, which can quickly release genomic DNA from plant seed samples with high polysaccharides and polyphenols (such as bananas, cotton, etc.) for use in PCR reactions, so it is particularly suitable for large-scale genetic testing. In order to meet the needs of different detection tests, this kit can use multiple types of samples (such as whole seeds, small tissue cuts, or ground samples of multiple seeds) for experiments. Among them, for the seed samples that still need to be germinated, you can choose to cut out micro-tissue pieces (1-5mg) other than the seed embryos for experiments; for experiments that require sampling and testing in a large number of samples, you can choose to mix multiple samples and combine them. After grinding into particles with a diameter of about 0.5mm, the experiment can be carried out (at least 0.1% of the target sample can be detected).

Specifications

50T,200T,500T,2000T

Kit components

Plant Seed Direct PCR Plus Kit II

Polysaccharide polyphenol plant seed (large and medium-sized) direct PCR kit

 Kit composition

 TP-0317T TP-03171

TP-03172

 TP-03173
50 T 200 T 500 T 2000 T
 

Part I

 Buffer SSP1 24ml 96ml

120ml×2

 480ml×2
Buffer SP2 3ml 10ml 25ml 100ml
6× DNA Loading Buffer 1.5ml 1.5ml 1.5ml 1.5ml×4
 

Part II

 Buffer SSP2 8ml 32ml 80ml 80ml×4
2× Seed PCR EasyTM Mix 500μl 1ml×2

1.7ml×3

 1.7ml×12
Manual 1 1 1 1 

Features and advantages

- No time-consuming and expensive DNA purification is required.

- The sample demand is small, just take a single seed.

- No special treatments such as grinding and crushing are required, and the operation is simple.

- The optimized PCR system enables PCR to have higher specificity and stronger tolerance to PCR reaction inhibitors.

Workflow

Plant Seed Direct PCR Plus Kit I(Small)-UNG

Storage conditions

1.Transportation

The whole process of low-temperature ice box transportation, to ensure that the kit Part II is in a state of <4 ℃.

 2.Storage

Part I of this kit should be stored at room temperature or 2-8°C.

Part II should be stored at -20°C.

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