Plant Total RNA Isolation Plus Kit
Kit Description:
Effectively remove DNA using DNA-Cleaning Column
Remove DNA without adding DNase
Simple—all operations are completed at room temperature
Fast—operation can be completed in 30 minutes
Safe—no organic reagent used
Specifications
50 Preps, 200 Preps
The kit uses the spin column and formula developed by Foregene, which can efficiently extract high-purity and high-quality total RNA from various plant tissues with high polysaccharides or polyphenols content. It provides the DNA-Cleaning column that can easily remove genomic DNA from the supernatant and tissue lysate. RNA-only column can effectively bind RNA. The kit can process a large number of samples at the same time.
The entire system does not contain RNase, so the purified RNA will not be degraded. Buffer PRW1 and Buffer PRW2 can ensure that the RNA obtained is not contaminated by protein, DNA, ions, and organic compounds.
Kit components
|
Buffer PSL1, Buffer PS, Buffer PSL2 |
|
Buffer PRW1, Buffer PRW2 |
|
RNase-Free ddH2O, DNA-Cleaning Column |
|
RNA-Only Column |
Features&advantages
■ Operation at room temperature (15-25℃) throughout the whole process, without ice bath and low temperature centrifugation.
■ Complete kit RNase-Free, no need to worry about RNA degradation.
■ Particularly suitable for the purification of RNA from plant samples of polysaccharides and polyphenols.
■ DNA-Cleaning Column specifically binds to DNA, so that the kit can remove genomic DNA contamination without adding DNase.
■ High RNA yield: RNA-only Column and unique formula can efficiently purify RNA.
■ Fast speed: easy to operate and can be completed within 30 minutes.
■ Safety: no organic reagent is required.
■ High quality: The purified RNA fragments are of high purity, free of protein and other impurities, and can meet various downstream experimental applications.
Product parameters
■ Downstream applications: first-strand cDNA synthesis, RT-PCR, molecular cloning, Northern Blot, etc.
■ Sample: Fresh or frozen plant tissues of polysaccharides and polyphenols
■ Dosage: 50mg plant tissue
■ Maximum RNA binding capacity of purification column: 80 μg
■ Elution volume: 50-200 μl
Kit application
It is suitable for the extraction and purification of total RNA from fresh or frozen plant tissue samples (especially fresh plant leaf tissue) with high polysaccharide and polyphenol content.
Work flow
Diagram
Plant Total RNA Isolation Kit Plus processed 50mg of fresh leaves of polysaccharides and polyphenols, and 5% purified RNA was tested by electrophoresis.
1: Banana
2: Ginkgo
3: Cotton
4: Pomegranate
Storage and Shelf life
This kit can be stored for 24 months under dry conditions at room temperature (15-25℃); if it needs to be stored for a longer time, it can be stored in 2–8℃.
Buffer PSL1 can be placed at 4℃for 1 month after adding β-mercaptoethanol (it is recommended to add it at the same time of experiment).
