QuickEasyᵀᴹ Real Time PCR Kit-Taqman
Kit Description:
◮The unique PCR optimization system makes 2×QuickEasyTM Real PCR Mix-Taqman more compatible.
◮Hot-start Foregene HS Taq Polymerase has higher amplification efficiency, higher amplification sensitivity, and higher amplification specificity.
◮2× QuickEasyTM Real PCR Mix -Taqman uses a unique system optimized by Foregene to improve the sensitivity and specificity of sequence-specific probe detection, which can be used for genotyping and copy number variation determination and obtain accurate results.
◮This product comes with ROX internal reference dye, which can be used to eliminate signal background and signal error between wells, which is convenient for customers to use in different types of quantitative PCR instruments.
Descriptions
The 2× QuickEasyTM Real PCR Mix-Taqman provided by the QuickEasyTM Real Time PCR Kit-Taqman kit is a new generation master mix system designed for Real Time PCR amplification reaction using specific fluorescent probes. Its core Foregene HS Taq DNA Polymerase is based on Antibody modification, used in conjunction with Foregene further optimized PCR system, has higher specificity and amplification efficiency. Compared with ordinary PCR mix, it has stronger amplification ability and lower mismatch rate. It can be used in fluorescence quantitative PCR reaction to reduce non-specific amplification and improve the accuracy of PCR. It can greatly improve product specificity and reaction sensitivity. At the same time, ROX was provided as an internal reference dye.
The QuickEasyTM Real Time PCR Kit-Taqman kit has a broad scope of application to the amount of template, and can obtain a good standard curve in different quantitative regions, and can accurately quantify and detect target genes with good repeatability and high confidence.
Specifications
200 Preps, 500 Preps, 1000 Preps, 2000 Preps
Kit components
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QuickEasyTM Real Time PCR Kit-Taqman (20μl system) |
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Kit contents |
QP-01121 |
QP-01122 |
QP-01123 |
QP-01124 |
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200 Preps |
500 Preps |
1000 Preps |
2000 Preps |
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2× QuickEasyTM Real PCR Mix-Taqman |
1 mL × 2 |
1.7 mL × 3 |
1.7 mL × 6 |
1.7 mL × 12 |
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20× ROX Reference Dye |
200 μL |
0.5 mL |
1 mL |
1 mL × 2 |
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DNase-Free ddH2O |
1.7 mL |
1.7 mL × 2 |
10 mL |
20 mL |
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IFU |
1piece |
1piece |
1piece |
1piece |
Features&advantages
■ The unique PCR optimization system makes 2×QuickEasyTM Real PCR Mix-Taqman more compatible.
■ Hot-start Foregene HS Taq Polymerase has higher amplification efficiency, higher amplification sensitivity, and higher amplification specificity.
■ 2× QuickEasyTM Real PCR Mix -Taqman uses a unique system optimized by Foregene to improve the sensitivity and specificity of sequence-specific probe detection, which can be used for genotyping and copy number variation determination and obtain accurate results.
■ This product comes with ROX internal reference dye, which can be used to eliminate signal background and signal error between wells, which is convenient for customers to use in different types of quantitative PCR instruments.
Kit application
■ Fluorescence quantitative PCR for template quantitative analysis;
■ Conventional PCR amplification;
■ Can be used for allele detection.
Storage and Shelf life
The kit should be stored at -20°C in the dark; if it is used frequently, it can also be stored at 4°C for a short period of time (use up within 10 days).
Real Time PCR primer design principles
Forward Primer and Reverse Primer
For Real Time PCR, primer design is very important. Primers are related to the specificity and efficiency of PCR amplification, and can be designed with reference to the following principles:
Primer length: 18-30bp.
GC content: 40-60%.
Tm value: Primer design software, such as Primer 5, can give the Tm value of the primer. The Tm values of the upstream and downstream primers should be as close as possible. The Tm calculation formula can also be used: Tm = 4 °C (G + C) + 2 °C (A + T). When performing PCR, a temperature below the primer Tm value of 5 °C is generally selected as the annealing temperature (the corresponding increase in the annealing temperature can increase the specificity of the PCR reaction).
Primers and PCR products:
Design primer PCR amplification product length is preferably 100-150bp.
Design primers in the secondary structural area of the template should be avoided as much as possible.
Avoid the formation of 2 or more complementary bases between the 3′ ends of upstream and downstream primers.
Primer 3′ terminal base can not be present with 3 additional consecutive G or C.
The primers themselves cannot have complementary structures, otherwise a hairpin structure will be formed, affecting PCR amplification.
ATCG should be distributed as evenly as possible in the primer sequence, and the 3′ terminal base should be avoided as T.
Appendix 1：Cell Direct RT-qPCR Kit component supplement pack
1.Cell Lysis Solution
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Kit components (24-well lysis system / well) |
DRT-01011-A1 |
DRT-01011-A2 |
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100 T |
500 T |
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Part I |
Buffer CL |
20 ml |
100 ml |
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Foregene Protease Plus II |
400 μl |
1 ml × 2 |
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Buffer ST |
1 ml × 2 |
10 ml |
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Part II |
DNA Eraser |
400 μl |
1 ml × 2 |
2.RT Mix
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Kit components (20 μl reaction system) |
DRT-01011-B1 |
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200 T |
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5× Direct RT Mix |
800 μl |
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RNase-Free ddH2O |
1.7 ml × 2 |
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Kit components (20 μl reaction system) |
DRT-01011-C1 |
DRT-01011-C2 |
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200 T |
1000 T |
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2× Direct qPCR Mix-SYBR |
1 ml × 2 |
1.7 ml × 6 |
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50× ROX Reference Dye |
40 μl |
200 μl |
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RNase-Free ddH2O |
1.7 ml |
10 ml |
