Direct RT-qPCR series
Direct PCR
What is Direct PCR
Direct PCR is the method of DNA amplification directly from blood, animal tissue, rat tail,cell,rat ear, zebra fish, fish eggs, plant leaves, seeds or other original biological tissue sample without performing DNA isolation and purification steps. Direct PCR technique can greatly reduce experimental time, and cost in genotyping and high-quantity projects. It also provides a better option when faced with the challenges of amplifying a very small quantity of sample where the purification step could potentially lead to sample loss.
FOREGENE’s PCR Mix has strong stress resistance, because of FOREGENE strength patented Taq enzyme( authorized patent: ZL20161034512.0 (China), EP3450560 (Europe), PCT/CN2017/082154 (US), patent 6894926 (Japan)). Its Taq enzyme uses DNA recombination technology to recombine the DNA binding structure region of the archaeal nucleic acid binding protein with the heat-resistant Taq DNA polymerase to construct a template DNA with increased affinity. An improved hot-start Taq that retains various functions of the original DNA polymerase.This Taq enzyme can bind to template DNA and initiate DNA synthesis in low concentration templates and complex environments.
For instance,it is as easy as adding your cell, plant (young leaf, root or seed) or animal sample to the buffer, and adding the unique master mix with specific primers in PCR tubes and running it through a thermal cycler. Kit efficiency is ideal for large-scale sample analysis where saving time is extremely important.
The PCR reaction system containing UNG enzyme and dUTP is selected according to the experimental requirements, which can effectively avoid the pollution caused by PCR amplification products.
Advantages of Direct PCR
|
|
Direct PCR |
Traditional method (RNA purification+ Coventional PCR) |
Material consumption |
Tissue consumption |
Less |
Much |
Template preparation |
Material type |
No grinding required |
Grinding samples |
Operation |
One-tube type,10min |
complicated operation, 1h |
|
Flux |
1-96 |
1-24 |
|
PCR reaction |
reaction system |
Optimized 2×PCR mix |
Dntp, MgCl2,10×PCR Buffer,Taq enzyme, |
Application
01 Identification of pathogenic microorganisms
02 Genetically modified identification, genotyping
03 Multiplex PCR / SNP detection / PCR-RFLP
04Sequencing/cloning
Product Series
Series |
Product Name |
Specifications |
Catalogue Number |
Storage Conditions |
Direct RT-qPCR Series |
200T |
DRT-01011 |
Part I 4℃,Part II -20℃ |
|
1000T |
DRT-01012 |
Series |
Product Name |
Specifications |
Catalogue Number |
Storage Conditions |
Blood Direct PCR Series |
200 ×20μl rxns |
TP-04111 |
-20℃ |
|
2000 ×20μl rxns |
TP-04113 |
|||
200 ×20μl rxns |
TP-04121 |
-20℃ |
||
2000 ×20μl rxns |
TP-04123 |
|||
200 ×20μl rxns |
TP-04211 |
-20℃ |
||
2000 ×20μl rxns |
TP-04213 |
|||
200 ×20μl rxns |
TP-04221 |
-20℃ |
||
2000 ×20μl rxns |
TP-04223 |
Series |
Product Name |
Specifications |
Catalogue Number |
Storage Conditions |
Animal Direct PCR Series |
Animal Tissue Direct PCR Kit(65℃ lysis) |
200 ×20μl rxns |
TP-01111 |
Part I 4℃,Part II -20℃ |
2000 ×20μl rxns |
TP-01113 |
|||
Animal Tissue Direct PCR Kit(65℃ lysis) -UNG |
200 ×20μl rxns |
TP-01121 |
Part I 4℃,Part II -20℃ |
|
2000 ×20μl rxns |
TP-01123 |
|||
Mouse Tail SuperDirectTM PCR Kit (room temperature lysis) |
200 ×20μl rxns |
TP-01311 |
Part I 4℃,Part II -20℃ |
|
2000 ×20μl rxns |
TP-01313 |
|||
Mouse Tail Direct PCR Kit(65℃ lysis) |
200 ×20μl rxns |
TP-01331 |
Part I 4℃,Part II -20℃ |
|
2000 ×20μl rxns |
TP-01333 |
|||
Mouse Tail Direct PCR Kit(65℃ lysis) - UNG |
200 ×20μl rxns |
TP-01341 |
Part I 4℃,Part II -20℃ |
|
2000 ×20μl rxns |
TP-01343 |
|||
Zebra Fish Direct PCR Kit(65℃ lysis) |
200 ×20μl rxns |
TP-01411 |
Part I 4℃,Part II -20℃ |
|
2000 ×20μl rxns |
TP-01413 |
|||
Zebra Fish Direct PCR Kit(65℃ lysis) - UNG |
200 ×20μl rxns |
TP-01421 |
Part I 4℃,Part II -20℃ |
|
2000 ×20μl rxns |
TP-01423 |
Series |
Product Name |
Specifications |
Catalogue Number |
Storage Conditions |
Plant Direct PCR Series |
200 ×20μl rxns |
TP-02111 |
Part I 4℃,Part II -20℃ |
|
2000 ×20μl rxns |
TP-02113 |
|||
200 ×20μl rxns |
TP-02121 |
Part I 4℃,Part II -20℃ |
||
2000 ×20μl rxns |
TP-02123 |
|||
Plant Leaf Direct PCR Plus Kit |
200 ×20μl rxns |
TP-02131 |
Part I 4℃,Part II -20℃ |
|
2000 ×20μl rxns |
TP-02133 |
|||
Plant Leaf Direct PCR Plus Kit -UNG |
200 ×20μl rxns |
TP-02141 |
Part I 4℃,Part II -20℃ |
|
2000 ×20μl rxns |
TP-02143 |
|||
200 ×20μl rxns |
TP-03111 |
Part I 4℃,Part II -20℃ |
||
2000 ×20μl rxns |
TP-03113 |
|||
200 ×20μl rxns |
TP-03121 |
Part I 4℃,Part II -20℃ |
||
2000 ×20μl rxns |
TP-03123 |
|||
200 ×20μl rxns |
TP-03131 |
Part I 4℃,Part II -20℃ |
||
2000 ×20μl rxns |
TP-03133 |
|||
200 ×20μl rxns |
TP-03141 |
Part I 4℃,Part II -20℃ |
||
2000 ×20μl rxns |
TP-03143 |
|||
Plant Seed Direct PCR Plus Kit I(Polysaccharide polyphenol plant) |
200 ×20μl rxns |
TP-03151 |
Part I 4℃,Part II -20℃ |
|
2000 ×20μl rxns |
TP-03153 |
|||
Plant Seed Direct PCR Plus Kit I - UNG(Polysaccharide polyphenol plant) |
200 ×20μl rxns |
TP-03161 |
Part I 4℃,Part II -20℃ |
|
2000 ×20μl rxns |
TP-03163 |
|||
200 ×20μl rxns |
TP-03171 |
Part I 4℃,Part II -20℃ |
||
2000 ×20μl rxns |
TP-03173 |
|||
Plant Seed Direct PCR Plus Kit II - UNG(Polysaccharide polyphenol plant) |
200 ×20μl rxns |
TP-03181 |
Part I 4℃,Part II -20℃ |
|
2000 ×20μl rxns |
TP-03183 |