Real Time PCR Easyᵀᴹ-SYBR Green I kit
Kit Description:
Simple—2X PCR Mix to reduce experimental error and operation time
Specific—optimized buffer and hot-start Taq enzyme can prevent non-specific amplification and primer dimer formation
High sensitivity—can detect low copies of template
Good versatility—compatible with most real-time quantitative PCR instruments
Kit Descriptions
The 2×Real PCR EasyTM Mix-SYBR provided by the Real Time PCR EasyTM-SYBR Green I kit is a new premix system that uses SYBR Green I for Real Time PCR amplification reactions, which can greatly improve product specificity and reaction sensitivity . ROX is provided as internal control dye. The fluorescence intensity of this kit is 3-5 times that of similar products, which can be more sensitive and intuitively reflect the concentration of target template DNA.
2×Real PCR EasyTM Mix-SYBR contains Foregene’s unique hot-start Taq DNA Polymerase. Compared with ordinary Taq enzymes, it has the advantages of high amplification efficiency, strong specific amplification ability and low mismatch rate. It can reduce non-specific amplification and improve the accuracy of PCR.
Specifications
|
Real Time PCR Easyᵀᴹ-SYBR Green I Kit |
||||
|
Kit composition(20μl system) |
QP-01011 |
QP-01012 |
QP-01013 |
QP-01014 |
|
200T |
500T |
1000T |
2000T |
|
|
2× Real PCR EasyTM Mix-SYBR |
1 ml × 2 |
1.7 ml × 3 |
1.7 ml × 6 |
1.7 ml × 12 |
|
50× ROX Reference Dye |
400 μl |
1 ml |
1 ml × 2 |
1 ml × 4 |
|
DNase-Free ddH2O |
1.7 ml |
1.7 ml × 2 |
10 ml |
20 ml |
|
Instruction |
1 |
1 |
1 |
1 |
Features&advantages
■ Simple—2X PCR Mix to reduce experimental error and operation time
■ Specific—optimized buffer and hot-start Taq enzyme can prevent non-specific amplification and primer dimer formation
■ High sensitivity—can detect low copies of template
■ Good versatility—compatible with most real-time quantitative PCR instruments
Kit application
qPCR analysis
Work flow
Graphic
Storage and shelf life
This kit should stored away from light and should be stored at -20℃. If used frequently, it can also be stored at 4℃ for a short period of time (10 days).
