RT Easy II(with gDNase) Master Premix for first-strand cDNA synthesis for Real Time PCR with gDNase
Kit Description:
-Efficient ability to remove gDNA, which can remove gDNA in the template within 2 minutes.
-Efficient reverse transcription system, it only takes 15 minutes to complete the synthesis of the first strand cDNA.
-Complex templates: templates with high GC content and complex secondary structure can also be reversed with high efficiency.
-High-sensitivity reverse transcription system, pg-level templates can also get high-quality cDNA.
-The reverse transcription system has high thermal stability, the optimal reaction temperature is 42℃, and it still has good reverse transcription performance at 50℃.
Specifications
25×20µl rxns,100×20µl rxns
RT EasyTM II (With gDNase) is a reverse transcription system specially developed for Real Time PCR to quickly remove genomic DNA contamination. 5×gDNase Mix can quickly remove the remaining genome in RNA at 42°C for 2 minutes, effectively avoiding the interference of genome on qPCR results.
5× RO-EasyTM Mix contains Foregene Reverse Transcriptase specially developed by Foregene, which is a new type of reverse transcriptase with stronger RNA affinity. The optimized system makes the reverse transcription rate faster, and the first strand cDNA synthesis can be completed at 42°C in 15 minutes.
The reverse transcription system of this kit can also maintain good activity at 50°C. Therefore, it is more advantageous for complex RNA templates and can easily transcribe RNA templates with high GC content and complex secondary structure.
Kit components
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5× gDNase Mix |
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5× RO-EasyTM Mix |
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RNase-Free ddH2O |
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Instructions |
Features&advantages
-Efficient ability to remove gDNA, which can remove gDNA in the template within 2 minutes.
-Efficient reverse transcription system, it only takes 15 minutes to complete the synthesis of the first strand cDNA.
-Complex templates: templates with high GC content and complex secondary structure can also be reversed with high efficiency.
-High-sensitivity reverse transcription system, pg-level templates can also get high-quality cDNA.
-The reverse transcription system has high thermal stability, the optimal reaction temperature is 42℃, and it still has good reverse transcription performance at 50℃.
Kit application
Used directly in Real Time PCR to quantitatively analyze gene expression.
It can quickly and accurately analyze trace RNA such as RNA virus.
Reverse transcription of RNA templates with high GC content or complex secondary structure.
Workflow
Storage and Shelf life
The kit should be stored at -20°C. Store the product in a constant temperature refrigerator at -20°C immediately after receipt. If the storage conditions are appropriate, the product will not degrade any performance during the 1-year validity period.
