RT-PCR Easyᵀᴹ I(One Step)
Kit Description:
- The one-step kit enables reverse transcription and PCR to be carried out in the same tube. It only needs to add template RNA, specific PCR primers and RNase-Free ddH2O.
- Real-time quantitative analysis of RNA can be carried out quickly and accurately.
- The kit uses a unique Foregene reverse transcription reagent and Foregene HotStar Taq DNA Polymerase combined with a unique reaction system to effectively improve the amplification efficiency and specificity of the reaction.
- The optimized reaction system makes the reaction have higher detection sensitivity, stronger thermal stability, and better tolerance.
Specifications
Foregene One-Step RT-PCR EasyTM series products realize the integrated reaction from RNA to double-stranded DNA, that is, reverse transcription and PCR amplification are completed in the same reaction centrifuge tube and the same reaction system, which simplifies the experimental steps, optimizes the experimental program, and improves the experiment efficiency.
RT-PCR EasyTM I(One Step) is a one-step RT-PCR kit specially developed by Foregene, which realizes continuous operation from RT to PCR in the same tube. The operation is simple and fast, which can effectively reduce the pollution during the experiment. 2×RT-PCR EasyTM Mix has strong tolerance, thermal stability, high efficiency and specificity for amplification.
Kit components
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RT-PCR EasyTM I(One Step) |
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Kit contents |
RT-02011 |
RT-02012 |
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100T (20μl system) |
500T (20μl system) |
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2× RT-PCR EasyTM Mix |
1ml |
1.7ml×3 |
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RNase-Free ddH2O |
1.7ml |
1.7ml×3 |
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Instruction Manual |
1 piece |
1piece |
Features&advantages
- The one-step kit enables reverse transcription and PCR to be carried out in the same tube. It only needs to add template RNA, specific PCR primers and RNase-Free ddH2O.
- Real-time quantitative analysis of RNA can be carried out quickly and accurately.
- The kit uses a unique Foregene reverse transcription reagent and Foregene HotStar Taq DNA Polymerase combined with a unique reaction system to effectively improve the amplification efficiency and specificity of the reaction.
- The optimized reaction system makes the reaction have higher detection sensitivity, stronger thermal stability, and better tolerance.
Workflow
Storage and Shelf life
1.Transportation conditions
The whole process of low-temperature ice box transportation, to ensure that the kit is in a state of <4 °C.
2. Storage conditions
The kit is stored at -20°C. Store the product in a constant temperature refrigerator at -20°C immediately after receipt. If storage conditions are appropriate,the product will not degrade any performance during the 1-year validity period.
