RT-PCR Easyᵀᴹ II(Two Step)
Specifications
RT-PCR EasyTM II(Two Step) contains all the reagents for two-step RT-PCR, combining RT reaction and PCR reaction in the same kit, providing optimized RT Mix and PCR Mix. The unique RT Mix can synthesize high-quality first-strand cDNA easily and efficiently. 2× PCR EasyTM Mix Plus has high-efficiency and specific amplification. The organic combination of the two makes the detection of the target gene more sensitive.
Kit components
RT-PCR EasyTM II(Two Step) |
||
Kit contents |
RT-02021 |
RT-02022 |
100T (20μl system) |
500T (20μl system) |
|
2× RT EasyTM Mix |
1ml |
1.7ml×3 |
2× PCR EasyTM Mix Plus |
1ml |
1.7ml×3 |
Random Primer (50μM) |
200μl |
1ml |
Oligo(dT)18 Primer (50μM) |
200μl |
1ml |
RNase-Free ddH2O |
1.7ml |
1.7ml |
Instruction Manual |
1 piece |
1 piece |
Features&advantages
- Real-time quantitative analysis of RNA can be carried out quickly and accurately.
- The kit uses a unique Foregene reverse transcription reagent and Foregene HotStar Taq DNA Polymerase combined with a unique reaction system to effectively improve the amplification efficiency and specificity of the reaction.
- The optimized reaction system makes the reaction have higher detection sensitivity, stronger thermal stability, and better tolerance.
Workflow
Storage and Shelf life
1.Transportation conditions
The whole process of low-temperature ice box transportation, to ensure that the kit is in a state of <4 °C.
2. Storage conditions
The kit is stored at -20°C. Store the product in a constant temperature refrigerator at -20°C immediately after receipt. If storage conditions are appropriate,the product will not degrade any performance during the 1-year validity period.