RT-qPCR Easyᵀᴹ (One Step)-Taqman
Kit Description:
- One-step kit enables reverse transcription and PCR to be carried out in the same tube, only need to add template RNA, specific PCR primers and RNase-Free ddH2O.
- Real-time quantitative analysis of viral RNA or trace RNA can be carried out quickly and accurately.
- The kit uses a unique Foregene reverse transcription reagent and Foregene HotStar Taq DNA Polymerase combined with a unique reaction system to effectively improve the amplification efficiency and specificity of the reaction.
- The optimized reaction system makes the reaction have higher detection sensitivity, stronger thermal stability, and better tolerance.
- RT-qPCR EasyTM (One Step)-Taqman kit comes with ROX internal reference dye, which can be used to eliminate signal background and signal errors between wells, which is convenient for end users to use in different models of quantitative PCR instruments
Descriptions
Foregene One-Step RT-PCR Easy series products realize the integrated reaction from RNA to double-stranded DNA, that is, reverse transcription and PCR amplification are completed in the same reaction centrifuge tube and the same reaction system, thus the experimental steps simplified, the experimental plan optimized, and the experimental efficiency improved.
RT-qPCR EasyTM (One Step)-Taqman using Foregene HotStar Taq DNA Polymerase, the optimized Taqman qPCR system can quickly and specifically perform Real Time RT-qPCR quantitative detection of trace RNA templates.
Specifications
100 Rxns (20μl system)
500 Rxns (20μl system)
Kit components
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RT-qPCR EasyTM (One Step)-Taqman |
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Kit components |
RT-02131 | RT-02132 |
| 100Rxns (20μl system) | 500 Rxns (20μl system) | |
| 2× RT-qPCR EasyTM Mix-Taqman | 1ml | 1.7ml×3 |
| 20× ROX Reference Dye | 100μl | 0.5ml |
| RNase-Free ddH2O | 1.7ml | 1.7ml×3 |
| Instruction For Use | 1 | 1 |
Features&advantages
- One-step kit enables reverse transcription and PCR to be carried out in the same tube, only need to add template RNA, specific PCR primers and RNase-Free ddH2O.
- Real-time quantitative analysis of viral RNA or trace RNA can be carried out quickly and accurately.
- The kit uses a unique Foregene reverse transcription reagent and Foregene HotStar Taq DNA Polymerase combined with a unique reaction system to effectively improve the amplification efficiency and specificity of the reaction.
- The optimized reaction system makes the reaction have higher detection sensitivity, stronger thermal stability, and better tolerance.
- RT-qPCR EasyTM (One Step)-Taqman kit comes with ROX internal reference dye, which can be used to eliminate signal background and signal errors between wells, which is convenient for end users to use in different models of quantitative PCR instruments
Kit parameters
Kit application
- This kit is used for rapid and accurate detection of trace RNAs such as RNA viruses.
- It is especially suitable for rapid detection of RNA templates with high GC content or complex secondary structure.
Workflow
Storage conditions
1. Transportation conditions
The whole process of low-temperature ice box transportation, to ensure that the kit is in a state of <4 ℃.
2. Storage conditions
The kit is stored at -20℃. Store the product in a constant temperature refrigerator at -20℃ immediately after receipt. If the storage conditions are appropriate, the product will not degrade any performance during the 1-year validity period.
