SARS-CoV-2 Nucleic Acid Detection Kit (Multiplex PCR Fluorescent Probe Method)
Kit Description:
◮ Direct PCR: No need for nucleic acid extaction kit and System, completed 96 specimen in 1 hour or so.
◮ Low equipment requirement and highly flexible use:Only need Real-Time PCR system .
◮ LoD and high sensitivity:Detection sensitivity can reach the lowest level as 500 copies/ml.
Intended Use
Principle of testing
This primer and probe sets are designed for the detection of the conserved sequences of SARS-CoV-2 (ORF1ab gene, N gene and E gene). The kit contains an Internal Control that is used to assess specimen quality. Real-Time PCR technology utilizes polymerase chain reaction (PCR) for the amplification of specific target sequences and target specific probes for the detection of the amplified RNA. The probes are labelled with fluorescent reporter and quencher dyes.
Quick Details
◮Specifications: 48 Rxns/kit, 96 Rxns/kit
◮ Specifificity:96.72%
Sensitivity:97.92%
Components
|
No. |
Component | Amount | Main ingredient | |||
| 48 Rxns | 96 Rxns | |||||
| 1 | Nucleic acid releaseagent | 1.5 mL/tube | 1 tube | 1.5 mL/tube |
1 tube |
Surfactant |
| 2 | RNA protectant | 27 μL/tube | 1 tube | 53 μL/tube |
1 tube |
RNase inhibitor |
| 3 | SARS-CoV-2reaction solution | 800 μL/tube | 1 tube | 1600 μL/tube |
1 tube |
Primer, probe, reaction buffer, dNTP |
| 4 | SARS-CoV-2enzyme mixture | 80 μL/tube | 1 tube | 160 μL/tube |
1 tube |
Hot start Taq enzyme,M-MLV enzyme |
| 5 | SARS-CoV-2positive control | 100 μL/tube | 1 tube | 100 μL/tube |
1 tube |
Recombinant plasmid containing targetfragment, RNA |
| 6 | SARS-CoV-2negative control | 1200 μL/tube | 1 tube | 1200 μL/tube |
1 tube |
TE buffer |
Features&advantages
How to use
Method 1: Direct PCR method
Method 2: Viral RNA Isolation + PCR
Method 1: Direct PCR method using Sample Release Agent
Detection procedure
Cold Chain Transportation and Storage
Sealed from light and stored at -20±5℃;
Shelf life
1 year
Ordering Information
