SARS-CoV-2 Variant Nucleic Acid Detection Kit III (Multiplex PCR Fluorescent Probe Method)-for detection of Delta mutants
Kit Description:
◮ Direct PCR: No need for nucleic acid extaction kit and System, completed 96 specimen in 1 hour or so.
◮ Low equipment requirement and highly flexible use:Only need Real-Time PCR system .
◮ LoD and high sensitivity:Detection sensitivity can reach the lowest level as 500 copies/ml.
Specifications
48 Rxns/kit, 96 Rxns/kit
|
No. |
Component |
Amount |
Main ingredient |
|||
|
48 Rxns |
96 Rxns |
|||||
|
1 |
Nucleic acid release agent |
1.4 mL/ tube |
2 tubes |
5.3 mL/bottle |
1 bottle |
Surfactant |
|
2 |
RNA protectant |
53 μL/tube |
1 tube |
106 μL/tube |
1 tube |
RNase inhibitor |
|
3 |
SARS-CoV-2 Variant reaction solution A |
800 μL/tube |
1 tube |
1600 μL/tube |
1 tube |
Primer, probe, reaction buffer, dNTP |
|
4 |
SARS-CoV-2 enzyme mixture |
160 μL/tube |
1 tube |
320 μL/tube |
1 tube |
Hot start Taq enzyme, M-MLV enzyme |
|
5 |
SARS-CoV-2 positive control |
100 μL/tube |
1 tube |
100 μL/tube |
1 tube |
Recombinant plasmid containing target fragment, RNA |
|
6 |
SARS-CoV-2 negative control |
1200 μL/tube |
1 tube |
1200 μL/tube |
1 tube |
TE buffer |
Features&advantages:
Quick and easy:complete the test in about 1 hour
Avoid nucleic acid loss:no need for nucleic acid purification
Low detection limit and high sensitivity, especially suitabe for large-scale rapid detection
Low equipment requirement and highly flexible use
Detection sensitivity can reach the lowest level as 500 copies/mL).
How to use:
1.Applicable instrument
ABI7500,Bio-Rad CFX96,Roche LightCycler 480,SLAN-96S
2.Detection procedure
Sample processing:Naopharyngeal or oropharyngeal swab sample (only 1 step operation)(Wait for 10 mins)
PCR amplificaton(<=55min)
Workflow:
Storage:
Sealed from light and stored at -20±5℃;
Store at 2-8℃ after opening, use within 7 days; avoid repeated freeze-thaw(<5 times)
Shelf life:1 year
