SARS-CoV-2/Flu/RSV Nucleic Acid Detection Kit (Multiplex PCR Fluorescent Probe Method)
Specifications
48 Rxns/kit, 96 Rxns/kit
No. |
Component |
Amount |
Main ingredient |
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48Rxns |
96Rxns |
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1 |
Nucleic acid release agent |
1.5 mL/ tube |
1 tube |
1.5 mL/ bottle |
1 bottle |
Surfactant |
2 |
RNA protectant |
53 μL /tube |
1 tube |
53 μL/ tube |
1 tube |
RNase inhibitor |
3 |
SFR reaction solution |
800 μL/ tube |
1 tube |
1600 μL/ tube |
1 tube |
Primer, probe, reaction buffer, dNTP |
4 |
enzyme mixture |
160 μL/ tube |
1 tube |
160 μL/ tube |
1 tube |
Hot start Taq enzyme, M-MLV enzyme |
5 |
SFR positive control |
100 μL/ tube |
1 tube |
100 μL/ tube |
1 tube |
Recombinant plasmid containing target fragment, RNA |
6 |
SFR negative control |
1200 μL/ tube |
1 tube |
1200 μL/ tube |
1 tube |
TE buffer |
Note:Components from different kits cannot be used interchangeably.
Features&advantages:
Quick and easy:complete the test in about 1 hour
Avoid nucleic acid loss:no need for nucleic acid purification
Low detection limit and high sensitivity, especially suitabe for large-scale rapid detection
Low equipment requirement and highly flexible use
Detection sensitivity can reach the lowest level as 500 copies/mL).
How to use:
1.Applicable instrument
ABI7500,Bio-Rad CFX96,Roche LightCycler 480,SLAN-96S
2.Detection procedure
Sample processing:Naopharyngeal or oropharyngeal swab sample (only 1 step operation)(Wait for 10 mins)
PCR amplificaton(<=55min)
Workflow:
Storage:
Sealed from light and stored at -20±5℃;
Store at 2-8℃ after opening, use within 7 days; avoid repeated freeze-thaw(<5 times)
Shelf life:1 year