SARS-CoV-2/Flu/RSV Nucleic Acid Detection Kit (Multiplex PCR Fluorescent Probe Method)

SARS-CoV-2/Flu/RSV Nucleic Acid Detection Kit (Multiplex PCR Fluorescent Probe Method)

Kit Description:

This kit uses Real-time RT PCR technology (rRT-PCR) for qualitative detection of SARS-CoV-2 FluA, FluB, RSVA and RSVB nucleic acids in human nasopharyngeal or oropharyngeal swab samples.

This is a multiplexed method that can facilitate detection and differentiation of SARS-CoV-2 and influenza viruses. Foregene’s assays can facilitate continued testing for both influenza and SARS-CoV-2 and can save both time and resources when customers head into influenza season.

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Product Detail

Product Tags

Specifications

48 Rxns/kit, 96 Rxns/kit

No.

Component

Amount

Main ingredient

48Rxns

96Rxns

1

Nucleic acid release agent

1.5 mL/ tube

1 tube

1.5 mL/ bottle

1 bottle

Surfactant

2

RNA protectant

53 μL /tube

1 tube

53 μL/ tube

1 tube

RNase inhibitor

3

SFR reaction solution

800 μL/ tube

1 tube

1600 μL/ tube

1 tube

Primer, probe, reaction buffer, dNTP

4

enzyme mixture

160 μL/ tube

1 tube

160 μL/ tube

1 tube

Hot start Taq enzyme, M-MLV enzyme

5

SFR positive control

100 μL/ tube

1 tube

100 μL/ tube

1 tube

Recombinant plasmid containing target fragment, RNA

6

SFR negative control

1200 μL/ tube

1 tube

1200 μL/ tube

1 tube

TE buffer

Note:Components from different kits cannot be used interchangeably.

Features&advantages:

Quick and easy:complete the test in about 1 hour

Avoid nucleic acid loss:no need for nucleic acid purification

Low detection limit and high sensitivity, especially suitabe for large-scale rapid detection

Low equipment requirement and highly flexible use

Detection sensitivity can reach the lowest level as 500 copies/mL).

How to use:

1.Applicable instrument

ABI7500,Bio-Rad CFX96,Roche LightCycler 480,SLAN-96S

2.Detection procedure

Sample processing:Naopharyngeal or oropharyngeal swab sample (only 1 step operation)(Wait for 10 mins)

PCR amplificaton(<=55min)

Workflow:

SARS-CoV-2 SARS-CoV-3 SARS-CoV-4

Storage:

Sealed from light and stored at -20±5℃;

Store at 2-8℃ after opening, use within 7 days; avoid repeated freeze-thaw(<5 times)

Shelf life:1 year


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