Viral DNA&RNA Isolation Kit Viral DNA and RNA Extraction Purification Preparation Kits
Specifications
50 Preps, 200 Preps
Viral RNA Nucleic acid Purification Extraction Isolation Kit uses the spin column and formula developed by Foregene, which can efficiently extract high-purity and high-quality viral RNA from samples such as plasma, serum, cell-free body fluid, and cell culture supernatant. The kit specifically adds Linear Acrylamide, which can easily capture small amounts of RNA from the samples. RNA-Only Column can efficiently bind RNA. The kit can process a large number of samples at the same time.
The entire kit does not contain RNase, so the purified RNA will not be degraded. Buffer viRW1 and Buffer viRW2 can ensure that the obtained viral nucleic acid free of protein, nuclease or other impurities, which can be used directly for downstream molecular biology experiments.
Kit components
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Linear Acrylamide |
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Buffer DRL |
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Buffer RW1, Buffer RW2 |
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RNase-Free ddH2O |
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DNA/RNA Column |
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Instructions |
Features&advantages
■ Operation at room temperature (15-25℃) throughout the whole process, without ice bath and low temperature centrifugation.
■ Complete kit RNase-Free, no need to worry about RNA degradation.
■ High nucleic acid yield: DNA/RNA-only Column and unique formula can efficiently purify DNA and RNA.
■ Large sample processing capacity: up to 200μl samples can be processed each time.
■ Fast speed: easy to operate and can be completed within 20 minutes.
■ Safety: no organic reagent is required.
■ High quality: The purified RNA fragments are of high purity, free of protein and other impurities, and can meet various downstream experimental applications.
Kit application
It is suitable for the extraction and purification of viral nucleic acid in samples such as plasma, serum, cell-free body fluid and cell culture supernatant.
Work flow
Diagram
Storage and Shelf life
■ This kit can be stored for 24 months under dry conditions at room temperature (15-25℃); if it needs to be stored for a longer time, it can be stored in 2–8℃.
■ Linear Acrylamide solution can be stored at room temperature for 7 days; after receiving the kit, please take it out and store it at -20°C.
■ After adding Linear Acrylamide to Buffer DRL, it can be stored at 2-8°C for up to 48h. Please use the ready-made solution.
Problem Analysis Guide
The following is an analysis of the problems that may be encountered in the extraction of viral DNA/RNA, hoping to be helpful to your experiments. In addition, for other experimental or technical problems other than operation instructions and problem analysis, we have dedicated technical support to help you. If you have any needs, please contact us: 028-83360257 or E-mail:
Tech@foregene.com.
No nucleic acid extraction or low nucleic acid yield
There are usually many factors that affect the recovery efficiency, such as: sample nucleic acid content, operation method, elution volume, etc.
Analysis of common causes:
1. An ice bath or low temperature (4°C) centrifugation was performed during the procedure.
Suggestion: Operate at room temperature (15-25°C) throughout the whole process, do not ice bath and low temperature centrifugation.
2. The sample was stored improperly or the sample was stored for too long.
Recommendation: Store samples at -80°C and avoid repeated freezing and thawing; try to use freshly collected samples for nucleic acid extraction.
3. Insufficient sample lysis.
Recommendation: Please ensure that the sample and lysis working solution are thoroughly mixed and incubated at room temperature (15-25°C) for 10 minutes.
4. Incorrect addition of eluent.
Suggestion: Make sure that RNase-Free ddH2O is added dropwise to the middle of the purification column membrane, and do not drop it on the purification column ring.
5. The correct volume of absolute ethanol was not added to Buffer RW2.
Suggestion: Please follow the instructions, add the correct volume of absolute ethanol to Buffer RW2 and mix well before using the kit.
6. Inappropriate sample volume.
Suggestion: 200µl of sample is processed for every 500µl of Buffer DRL. Excessive sample processing will result in lower nucleic acid extraction yield.
7. Inappropriate elution volume or incomplete elution.
Recommendation: The eluent volume of the purification column is 30-50μl; if the elution effect is not satisfactory, it is recommended to extend the time at room temperature after adding preheated RNase-Free ddH2O, such as 5-10min.
8. Ethanol remains on the column after washing with Buffer RW2.
Suggestion: If ethanol remains after centrifugation with Buffer RW2 for 2 minutes, the column can be placed at room temperature for 5 minutes after centrifugation to fully remove residual ethanol.
The purified nucleic acid is degraded
The quality of the purified nucleic acid is related to the preservation of the sample, RNase contamination, operation and other factors. Analysis of common causes:
1. The collected samples were not stored in time.
Suggestion: If the sample is not used in time after collection, please store it at -80°C at low temperature immediately. For RNA extraction, try to use freshly collected samples.
2. Collect samples and freeze and thaw repeatedly.
Suggestion: Avoid freezing and thawing (not more than once) during the collection and storage of samples, otherwise the nucleic acid yield will be reduced.
3. RNase is introduced in the operation room or disposable gloves, masks, etc. are not worn.
Recommendation: RNA extraction experiments are best performed in a separate RNA operation room, and the laboratory table should be cleaned before the experiment.
Wear disposable gloves and masks during the experiment to avoid RNA degradation caused by the introduction of RNase to the greatest extent.
4. The reagent was contaminated with RNase during use.
Recommendation: Replace with a new Viral DNA/RNA Isolation Kit for related experiments.
5. The centrifuge tubes and pipette tips used for RNA manipulation are contaminated with RNase.
Suggestion: Make sure that the centrifuge tubes, pipette tips, pipettes, etc. used for RNA extraction are all RNase-Free.
Purified nucleic acid affects downstream experiments
DNA and RNA purified by the purification column, if the salt ion and protein content are too high, it will affect the downstream experiments, such as: PCR amplification, reverse transcription, etc.
1. The eluted DNA and RNA have residual salt ions.
Suggestion: Make sure that the correct volume of absolute ethanol is added to Buffer RW2, and wash the purification column twice at the centrifugation speed specified in the operating instructions; Perform centrifugation to minimize salt ion contamination.
2. The eluted DNA and RNA have ethanol residues.
Suggestion: After confirming the washing with Buffer RW2, perform empty tube centrifugation at the centrifugation speed in the operating instructions; if there is still ethanol residue, you can centrifuge the empty tube and then place it at room temperature for 5 minutes to remove the ethanol residue to the greatest extent.
Instruction Manuals:
Viral DNA&RNA Isolation Kit Instruction Manual
