China Zebra Fish Direct PCR Kit-UNG Direct PCR Lysis Reagent(zebrafish) Manufacturer and Supplier

Zebra Fish Direct PCR Kit-UNG Direct PCR Lysis Reagent(zebrafish)

Kit Description:

Direct use of zebrafish lysate as a template for PCR amplification, no separate extraction of DNA, fast and high sensitivity. Added UNG anti-pollution system to eliminate false positives.

◮No time-consuming and expensive DNA purification is required.

◮The sample demand is small, just take a single seed.

◮No special treatments such as grinding and crushing are required, and the operation is simple.

◮The optimized PCR system enables PCR to have higher specificity and stronger tolerance to PCR reaction inhibitors.

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Product Detail

Product Tags

Descriptions

This kit uses a unique lysis buffer system to quickly release genomic DNA from zebrafish and other freshwater fish tissues, tail fins or fish eggs samples for PCR reactions, so it is particularly suitable for large-scale genetic testing. The process of releasing genomic DNA from the lysis buffer is completed within 10-30 minutes at 65°C. No other processes such as protein and RNA removal are required, and the released trace DNA can be used as a template for PCR reaction.

2× PCR EasyTM Mix (UNG) uses dUTP instead of dTTP on the basis of 2× PCR EasyTM Mix, and adds UNG enzyme (Uracil-N-glycosylase) that can degrade the template containing dUTP at the same time. Before the PCR reaction, the UNG enzyme is used to degrade the PCR product containing uracil. The UNG enzyme will not have any effect on the template that does not contain uracil, thereby ensuring the specificity and accuracy of amplification and preventing the possibility of large-scale genetic testing. Contamination of PCR products occurred.

D-Taq DNA polymerase is a DNA polymerase specially developed by Foregene for direct PCR reactions. D-Taq DNA polymerase has strong tolerance to a variety of PCR reaction inhibitors, and can efficiently amplify trace amounts of DNA in various complex reaction systems, and the amplification speed can reach 2Kb/min, which is especially suitable for Direct PCR reaction.

Specifications

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Kit components

Part I	Buffer FP
	Foregene Protease
	6× DNA Loading Buffer
Part II	2×PCR Easy^TM Mix(UNG)
Instructions

Features&advantages

■ No time-consuming and expensive DNA purification is required.

■ The sample demand is small, as little as 1mg fish caudal fin or 10 fish eggs can be tested.

■ No special treatments such as grinding and crushing are required, and the operation is simple.

■ Anti-pollution PCR system 2× PCR EasyTM Mix (UNG), which can effectively eliminate the pollution caused by PCR products and ensure the specificity and accuracy of amplification.

■ Optimized PCR system makes PCR have higher specificity and stronger tolerance to PCR reaction inhibitors.