Specifications

5ml, 10ml, 40ml

PCR Hero (With Dye)  Extraction kit includes Foregene’s unique new generation of Taq—Foregene Taq Hero, optimized dNTPs, MgCl2, reaction buffer, PCR reaction enhancer, optimizer and stabilizer. The 2× PCR HeroTM Mix (dye) system has higher tolerance to PCR inhibitors than the ordinary PCR Mix system, and can easily cope with PCR amplification of various complex templates. The unique reaction system and high-efficiency Taq Hero make the PCR reaction have higher amplification efficiency, specificity and sensitivity.

Kit components

Features&advantages

■  Higher fidelity: 6 times that of ordinary Taq enzyme;

■ Faster amplification speed : the amplification speed is 5-10sec/kb, which is 6-12 times that of ordinary Taq enzyme;

■ More template adaptability: It can efficiently amplify various complex DNA templates with high GC content;

■ Higher amplification efficiency: lower amplification cycles than ordinary Taq enzyme;

■ Environmental tolerance is stronger: placed at 37°C for a week, maintaining more than 90% activity;

■ It has 5′ →3′ DNA polymerase activity and 5′→3′ exonuclease activity, without 3′ →5′ exonuclease activity.

■ The kit is pre-mixed with Electrophoresis Loading Buffer, after PCR is completed, the sample can be directly loaded for electrophoresis; the operation is simple and easy.

Kit application

PCR amplification of DNA fragments, DNA labeling, DNA sequencing,PCR plus A tail

Storage and Shelf life

It can be stored for a long time at -20°C; for frequent use, it can be stored at 4°C for a short time.