Animal Total RNA Isolation Kit

Animal Total RNA Isolation Kit

Kit Description:

No need to worry about RNA degradation. The whole system is RNase-Free

Effectively remove DNA using DNA-Cleaning Column

Remove DNA without adding DNase

Simple—all operations are completed at room temperature

Fast—operation can be completed in 30 minutes

Safe—no organic reagent used

High purity—OD260/280≈1.8-2.1

 

20+ years R&D Manufacturing Experience     25+ highest impact factor                

1000+ different lab reagents                           100+ universities partner

400+ distributors                                             2000+ SCI citations of the lab reagents


Product Detail

Product Tags

Kits Descriptions

50 Preps, 200 Preps

This kit uses the spin column and formula developed by our company, which can extract high-purity and high-quality total RNA from various animal tissues with high efficiency.It provides an efficient DNA-Cleaning Column, which can easily separate and adsorb genomic DNA from the supernatant and tissue lysate, simple and time-saving; RNA-only Column can efficiently bind RNA and can be processed simultaneously with a unique formula Lots of samples.

The whole system is RNase-Free, so that the extracted RNA is not degraded; Buffer RW1, Buffer RW2 buffer washing system, so that the obtained RNA is free of protein, DNA, ion, and organic compound pollution.

 

Kit components:

Buffer RL1 , Buffer RL2
Buffer RW1, Buffer RW2
RNase-Free ddH2O, DNA-Cleaning Column
RNA-Only Column

Features&advantages

■ No need to worry about RNA degradation; the whole system is RNase-Free
■ Effectively remove DNA-using DNA-Cleaning Column
■ Remove DNA without adding DNase
■ Simple-all operations are completed at room temperature
■ Fast -operation can be completed in 30 minutes
■ Safe-no organic reagent required
■ High purity -OD260/280≈1.8-2.1

123

Kit application

It is suitable for the extraction and purification of total RNA from a variety of fresh or frozen animal tissues or cultured cells.

Product parameters

■ Downstream applications: first-strand cDNA synthesis, RT-PCR, molecular cloning, Northern Blot, etc.
■ Samples: animal tissues, cultured cells
■ Dosage: Tissues 10-20mg, Cells(2-5)×106
■ Maximum DNA binding capacity of purification column: 80 μg
■ Elution volume: 50-200 μl

Work flow

animal total RNA-simple workflow

Diagram

3 Animal Total RNA Isolation Kit7

Animal Total RNA Isolation Kit treated 20mg
Fresh mouse samples, take 5% purified total RNA 1% agar

Glycogel electrophoresis
1: Spleen 2: Kidney
3: Liver 4: Heart

Storage and shelf life

The kit can be stored for 24 months at room temperature (15–25 ℃) or 2–8 ℃ for longer time. Buffer RL1 can be stored at 4 ℃ for 1 month after adding β- mercaptoethanol (optional).


  • Previous:
  • Next:

  • Write your message here and send it to us

    Product categories