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Animal Total RNA Isolation Kit Total RNA Extraction and Purification Kits for Animal Tissues & Cell

Kit Description:

Quickly and efficiently extract high-purity and high-quality total RNA from various animal tissues.

No need to worry about RNA degradation. The whole system is RNase-Free

Effectively remove DNA using DNA-Cleaning Column

Remove DNA without adding DNase

Simple—all operations are completed at room temperature

Fast—operation can be completed in 30 minutes

Safe—no organic reagent used

High purity—OD260/280≈1.8-2.1

foregene strength


Product Detail

Product Tags

FAQ

Kits Descriptions

50 Preps, 200 Preps

This kit uses the spin column and formula developed by our company, which can extract high-purity and high-quality total RNA from various animal tissues with high efficiency.It provides an efficient DNA-Cleaning Column, which can easily separate and adsorb genomic DNA from the supernatant and tissue lysate, simple and time-saving; RNA-only Column can efficiently bind RNA and can be processed simultaneously with a unique formula Lots of samples.

The whole system is RNase-Free, so that the extracted RNA is not degraded; Buffer RW1, Buffer RW2 buffer washing system, so that the obtained RNA is free of protein, DNA, ion, and organic compound pollution.

Kit components

Animal Total RNA Isolation Kit
     Kit components RE-03011 RE-03014
50 T 200 T
Buffer RL1* 25ml 100ml
Buffer RL2 15ml 60ml
Buffer RW1* 25ml 100ml
Buffer RW2 24ml 96ml
RNase-Free ddH2O 10ml 40ml
RNA-only Column 50 200
DNA-Cleaning Column 50 200
Instruction Manual 1 piece 1 piece

 

Product information

Format Spin column Purification component Foregene column, reagent
Flux 1-24 samples Time per prep ~30 min (24 samples)
Centrifuge Desk centrifuge Pyrolysis separation Centrifugal separation
Sample Animal tissue; cell Samples amount Tissue:10-20 mg; Cell:(1-5)×106
Elution volume 50-200 μL Maximum loading volume 850 μL

 

Features&advantages

■ No need to worry about RNA degradation; the whole system is RNase-Free
■ Effectively remove DNA-using DNA-Cleaning Column
■ Remove DNA without adding DNase
■ Simple-all operations are completed at room temperature
■ Fast -operation can be completed in 30 minutes
■ Safe-no organic reagent required
■ High purity -OD260/280≈1.8-2.1

advantages of foregene RNA Isolation kit

Kit application

It is suitable for the extraction and purification of total RNA from a variety of fresh or frozen animal tissues or cultured cells.

Product parameters

■ Downstream applications: first-strand cDNA synthesis, RT-PCR, molecular cloning, Northern Blot, etc.
■ Samples: animal tissues, cultured cells
■ Dosage: Tissues 10-20mg, Cells(2-5)×106
■ Maximum DNA binding capacity of purification column: 80 μg
■ Elution volume: 50-200 μl

animal total RNA-simple workflow

Diagram

Animal Total RNA Isolation Kit treated 20mg
Fresh mouse samples, take 5% purified total RNA 1% agar

Glycogel electrophoresis
1: Spleen 2: Kidney
3: Liver 4: Heart

Storage and shelf life

The kit can be stored for 24 months at room temperature (15–25 ℃) or 2–8 ℃ for longer time. Buffer RL1 can be stored at 4 ℃ for 1 month after adding β- mercaptoethanol (optional).

Cited articles

1. IF:18.808: Zheng, Q., Qin, F., Luo, R., et al. mRNA-Loaded Lipid-Like Nanoparticles for Liver Base Editing Via the Optimization of Central Composite Design. Adv. Funct. Mater. 2021, 31, 2011068.doi:10.1002/adfm.202011068.

2.IF:18.187:He X, Hong W, Yang J, et al. Spontaneous apoptosis of cells in therapeutic stem cell preparation exert immunomodulatory effects through release of phosphatidylserine. Signal Transduct Target Ther. 2021 Jul 14;6(1):270. doi: 10.1038/s41392-021-00688-z.

3.IF:17.97:Dai Z, Liu H, Liao J, et al. N7-Methylguanosine tRNA modification enhances oncogenic mRNA translation and promotes intrahepatic cholangiocarcinoma progression. Mol Cell. 2021 Jul 29:S1097-2765(21)00555-4. doi: 10.1016/j.molcel.2021.07.003.

4.IF:9.225:Cao X, Shu Y, Chen Y, et al. Mettl14-Mediated m6A Modification Facilitates Liver Regeneration by Maintaining Endoplasmic Reticulum Homeostasis. Cell Mol Gastroenterol Hepatol. 2021;12(2):633-651. doi: 10.1016/j.jcmgh.2021.04.001.

 

RNA isoaltion kits for other sample sources are available:

Cell, plant, viral, blood, etc.


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  • RNA is not extracted or RNA yields are low

    There are often a variety of factors that affect recovery efficiency, such as: tissue sample RNA content, method of operation, elution volume, etc.

    1. Ice bath or cryogenic (4 °C) centrifugation was performed during operation.

    Recommendation: Operate at room temperature (15-25 ° C) throughout the whole process, do not ice bath and centrifuge at low temperatures.

    2. Improper sample preservation or excessive sample storage time.

    Recommendation: Store samples at -80 °C or freeze in liquid nitrogen and avoid repeated freeze-thaw use; try to use fresh tissue or cultured cells for RNA extraction.

    3. Insufficient sample lysis.

    Recommendation: When homogenizing tissue, ensure that the tissue is sufficiently homogenized and that the tissue cells are sufficiently split to explain the release of RNA.

    4. The eluent is not added correctly.

    Recommendation: Confirm that RNase-Free ddH2O is added dropwise to the middle of the purification column membrane.

    5. The correct volume of absolute ethanol was not added to Buffer RL2 or Buffer RW2.

    Recommendation: Follow the instructions, add the correct volume of absolute ethanol to Buffer RL2 and Buffer RW2 and mix well before using the kit.

    6. Tissue sample dosage is not appropriate.

    Recommendation: Use 10-20 mg of tissue or (1-5) × 106 cells per 500 μl buffer RL1, as excessive tissue use can result in reduced RNA extraction.

    7. Improper elution volume or incomplete elution.

    Recommendation: The elution volume of the purification column is 50-200 μl; if the elution effect is not satisfactory, it is recommended to extend the room temperature placement time after adding preheated RNase-Free ddH2O, e.g. for 5-10 min.

    8.The purification column has ethanol residue after Buffer RW2 wash.

    Recommendation: If there is ethanol residue after Buffer RW2 washing, empty tube centrifugation for 1min, the time for the empty tube centrifugation operation can be increased to 2min, or the purification column can be placed at room temperature for 5 min to adequately remove the residual ethanol.

    Purified RNA is degraded

    The quality of the purified RNA is related to factors such as the preservation of the sample, RNase contamination, and manipulation,etc.

    1. Tissue samples are not kept in time.

    Recommendation: If tissue samples or cells are not used in a timely manner after collection, immediately cryopreserve at -80 °C or liquid nitrogen. To extract RNA, use a newly taken tissue or cell sample whenever possible.

    2. Repeated freeze-thawing of tissue samples.

    Recommendation: When storing tissue samples, it is best to cut them into small pieces for preservation, and remove one of the pieces when using them to avoid repeated freeze-thawing of the sample and the degradation of RNA.

    3. RNase is introduced or not wearing disposable gloves, masks, etc. during the operation.

    Recommendation: RNA extraction experiments are best performed in separate RNA manipulation rooms and the table is cleared before the experiment.

    Wear disposable gloves and masks during the experiment to minimize RNA degradation caused by the introduction of RNase.

    4. Reagents are contaminated with RNase during use.

    Recommendation: Replace with a new Animal Total RNA Isolation Kit for related experiments.

    5. The centrifuge tubes, tips, etc. used in RNA manipulation are contaminated with RNase.

    Recommendation: Confirm that the centrifuge tubes, tips, pipettes, etc. used in RNA extraction are all RNase-Free.

    Purified obtained RNA affects downstream experiments

    RNA purified by the purification column, if the salt ions, protein content is too large will affect the downstream experiment, such as: reverse transcription,Northern Blot et al.

    1. The elutioned RNA has salt ion residues.

    Recommendation: Confirm that the correct volume of ethanol has been added to Buffer RW2 and perform 2 purification column washes at the centrifugal speed indicated for operation; if there is any salt ion residue, leave the purification column to Buffer RW2 for 5 min at room temperature and perform centrifugation to maximize the removal of salt contamination.

    2. Ethanol residue in elutioned RNA.

    Recommendation: Confirm that after buffer RW2 washing, perform the empty tube centrifugation operation at the centrifugation speed indicated for operation, increase the time of the empty tube centrifugation operation to 2 min if there is still ethanol residue, or leave it at room temperature for 5 min after the empty tube centrifugation to maximize the removal of ethanol residue.

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