For cell direct RT-qPCR using 10-1,000,000 cells,
and performing RT-qPCR directly from cells without prior RNA purification.
Cells are lysed directly to release RNA for RT-qPCR; the high tolerance system makes it unnecessary to purify RNA and directly use cell lysates as RNA templates for RT reactions. Fast and convenient; high sensitivity, strong specificity, and good stability.
◮Simple and effective: with Cell Direct RT technology, RNA samples can be obtained in just 7 minutes.
◮The sample demand is small, as low as 10 cells can be tested.
◮High throughput: it can quickly detect RNA in cells cultured in 384, 96, 24, 12, 6-well plates.
◮DNA Eraser can quickly remove released genomes, greatly reduce the impact on subsequent experimental results.
◮Optimized RT and qPCR system makes the two-step RT-PCR reverse transcription more efficient and PCR more specific, and more resistant to RT-qPCR reaction inhibitors.