Foregene DNA Identification System 27Y
Kit Description:
Foregene DNA Identification System 27Y uses six-color fluorescent labeling technology to amplify 27 Y-STR loci at one time. Including: DYS456, YGATAH4, DYS439, DYS19, DYS392, DYS576, DYS627, DYS391, DYS437, DYS570, DYS635, DYS448, DYS533, DYF387S1, DYS393, DYS389I, DYS390 , DYS389II, DYS438, DYS518, DYS460, DYS458, DYS481, DYS385, DYS449.
The kit has good anti-inhibition ability, and can directly amplify filter paper, FTA card, cotton swab, saliva card, oral swab, and can also be used to extract and purify DNA templates from case materials.
Description
Foregene DNA Identification System 27Y uses six-color fluorescent labeling technology to amplify 27 Y-STR loci at one time. Including: DYS456, YGATAH4, DYS439, DYS19, DYS392, DYS576, DYS627, DYS391, DYS437, DYS570, DYS635, DYS448, DYS533, DYF387S1, DYS393, DYS389I, DYS390 , DYS389II, DYS438, DYS518, DYS460, DYS458, DYS481, DYS385, DYS449.
The kit has good anti-inhibition ability, and can directly amplify filter paper, FTA card, cotton swab, saliva card, oral swab, and can also be used to extract and purify DNA templates from case materials.
Kit Content
|
Reagent test kit |
Component name |
Volume (μl/tube) |
Quantity (tube) |
|
PCR pre-reaction kit |
2×PCR Reaction Master Mix |
1250 |
2 |
| 5 x 27Y Primer Mix |
500 |
2 |
|
| DNA Typing Standard 9948 |
20 |
2 |
|
| Deionized water |
1700 |
2 |
|
|
Post-PCR Reaction Kit |
27Y allele mixture Ladder |
20 |
2 |
| Orange molecular weight internal standard ORG500 |
150 |
2 |
Data Analysis
1. Open the Gene Mapper ID software. When using this kit for the first time, you need to import panels, bin set, corresponding analysis method, and size standard ( ORG500 : 65, 70, 80, 100, 120 , 140, 160, 180, 200, 225, 250, 275, 300, 330, 360, 390, 420, 450, 490, 500).
2. Import the electrophoresis data, select the corresponding analysis parameters such as panel, analysis method, and size standard, and change the sample type of Ladder to "Allelic Ladder" in the "Sample Type" column; start analyzing the data.
Reagent Storage
1. Please store them below -20°C (if not used temporarily), after receiving the kits frozen in dry ice or gel ice packs.
2. Please store the kit at 4°C before the reaction to avoid repeated freezing and thawing, after the kit is taken out for use, ; the enzyme should be stored at - 20°C.
2. After the reaction, the kit (including molecular weight internal standard and ladder) should be stored in the "electrophoresis detection room" at 4°C, avoid repeated freezing and thawing, and avoid contact with the pre-reaction kit to avoid contamination.
