Foregene DNA Identification System 20A (Free DNA Extraction)
Kit Description:
Foregene DNA identification system 20A uses five-color fluorescent labeling technology to simultaneously amplify 19 STR loci and 1 sex loci in a single tube. While covering 13 CODIS core loci, 20 core loci meet the requirements of the Ministry of Public Security for autosomal STR kit. He is the right-hand man of forensic DNA database construction and judicial kinship identification.
Description
Foregene DNA identification system 20A uses five-color fluorescent labeling technology to simultaneously amplify 19 STR loci and 1 sex loci in a single tube. While covering 13 CODIS core loci, 20 core loci meet the requirements of the Ministry of Public Security for autosomal STR kit. He is the right-hand man of forensic DNA database construction and judicial kinship identification.
Kit Content
|
Kit Componet |
Componet Name |
Volume（μl /tube） |
Quantity（tube） |
|
PCR Pre-reaction reagent |
2.5×PCR reaction buffer Ⅲ |
1000 |
2 |
|
5×20A primer mix |
500 |
2 |
|
|
Control DNA 9947A（1ng/μl） |
25 |
1 |
|
|
Deionized water |
1700 |
2 |
|
|
Taq Polymerase |
Taq polymerase Ⅲ |
40 |
2 |
|
PCR Rear-reaction reagent |
Allelic ladder20A |
40 |
1 |
|
Molecular weight internal standardORG 500 |
150 |
2 |
|
|
Note |
|||
Data Analysis
1. Open the Genemapper ID software. When using this kit for the first time, you need to import the panels, bin set, corresponding analysis method, and size standard (ORG500: 65, 70, 80, 100, 120, 140, 160, 180, 200, 225, 250 , 275, 300, 330, 360, 390, 420, 450, 490, 500)
2. Import the electrophoresis data, select the corresponding analysis parameters such as panel, analysis method, and size standard, and change the sample type of Ladder to "Allelic Ladder" in the "Sample Type" column; start analyzing data.
Reagent Storage
1. After receiving the kit frozen in dry ice or plastic ice packs, if it is not used temporarily, please store it below -20°C for a long time.
2. After the kit is taken out and used, the kit should be stored at 4°C before the reaction to avoid repeated freezing and thawing; Taq enzyme should be stored at -20°C.
3. After the reaction, the kit (including molecular weight internal standard and ladder) should be stored in the "electrophoresis detection room" at 4°C, avoid repeated freezing and thawing, and avoid contact with the pre-reaction kit to avoid contamination.
