Foregene DNA Identification System 30A (Non-Extraction)
Kit Description:
FOREGENE DNA identification system 30A uses six-color fluorescent labeling technology to amplify 29 autosomal STR loci , one Amel locus and one Yindel locus at one time, and can directly amplify filter paper, FTA cards, cotton swabs, saliva cards, and mouth swab.
Description
FOREGENE DNA identification system 30A uses six-color fluorescent labeling technology to amplify 29 autosomal STR loci , one Amel locus and one Yindel locus at one time, and can directly amplify filter paper, FTA cards, cotton swabs, saliva cards, and mouth swab.
Kit Content
Amplification System Preparation
Table 1: The prepartion composition of Standard (Extraction-free) amplification reaction
|
|
Components |
25 μl system (μl ) |
10 μl system (μl ) |
|
Master Mix |
4 × Master Mix VII |
6.25 |
2.5 |
|
5 x 30A Primer Mix |
5.0 |
2 |
|
|
Deionized water |
13.75 |
5.5 |
|
|
Blood stain |
Diameter 1.2mm |
Diameter 1.2mm |
|
|
Total reaction volume |
25 μl |
10 μl |
|
Data Analysis
Annexed Graph 1: Allelic Ladder Typing Map
Annexed Graph 2: DNA typing standard 9948 typing map
Reagent Storage
1. Please store below -20°C,,after receiving the kits frozen in dry ice or gel ice packs.
2. Please store the pre-reaction component 4×premix VII at -20°C, after the kit taken out for using , and store the other remaining pre-reaction reagents at 4°C to avoid repeated freezing and thawing. If the single dosage is small, it is recommended to store at -20°C after aliquoting,
3. Store the components after the reaction at 4°C, avoid repeated freezing and thawing, and do not touch the reagents before the reaction to avoid contamination.
