Foregene DNA Identification System 30 A-C (Case)
Description
Foregene DNA identification system 30A-C uses six-color fluorescent labeling technology to amplify 29 autosomal STR loci , 1 Amel locus, 1 Yindel locus, and 1 human species-specific Sexual sites, 2 internal quality control sites, used to amplify the DNA template extracted and purified from Case materials.
Kit Content
Amplification system preparation
Table 2: The Preparation Composition of Standard extraction and amplification system
|
Components |
25ul system ( µl ) |
10μl system (μl) |
Master Mix |
2.5× Premix CⅡ |
10.0 |
4.0 |
5 x 30 AC Primer Mix |
5.0 |
2.0 |
|
DNA template |
2.5— 10 |
1— 4 |
|
Deionized water |
Make up to a reaction volume of 25 μl |
Make up to a reaction volume of 10 μl |
Data Analysis
Annexed Graph 1: Allelic Ladder Typing Map
Annexed Graph 2: DNA typing standard 9948 typing map
Reagent Storage
1. Please store below -20°C,,after receiving the kits frozen in dry ice or gel ice packs.
2. Please store the pre-reaction component 2.5× premix CII at -20°C after the kit is taken out for use,, and the remaining pre-reaction reagents are stored at 4°C to avoid repeated freezing and thawing. If the single dosage is small, it is recommended to store at -20°C after aliquoting.
3. Store the components after the reaction at 4°C, avoid repeated freezing and thawing, and do not touch the reagents before the reaction to avoid contamination.