Cell Direct RT qPCR Kit

Cell Direct RT qPCR Kit

Kit Description:

-Simple and effective : with Cell Direct RT technology, RNA samples can be obtained in just 7 minutes.

-The sample demand is small, as low as 10 cells can be tested.

-High throughput: it can quickly detect RNA in cells cultured in 384, 96, 24, 12, 6-well plates.

-DNA Eraser can quickly remove released genomes, greatly reduce the impact on subsequent experimental results.

-Optimized RT and qPCR system makes the two-step RT-PCR reverse transcription more efficient and PCR more specific, and more resistant to RT-qPCR reaction inhibitors.

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Product Detail

Product Tags

Descriptions

This kit uses a unique lysis buffer system that can quickly release RNA from cultured cell samples for RT-qPCR reactions, thereby eliminating the time-consuming and laborious RNA purification process. The RNA template can be obtained in just 7 minutes. The 5×Direct RT Mix and 2×Direct qPCR Mix-SYBR reagents provided by the kit can quickly and effectively obtain real-time quantitative PCR results.

5×Direct RT Mix and 2×Direct qPCR Mix-SYBR have strong inhibitor tolerance, and the lysate of the samples can be used as the template for RT-qPCR directly. This kit contains the unique RNA high-affinity Foregene reverse transcriptase, and Hot D-Taq DNA polymerase, dNTPs, MgCl2, reaction buffer, PCR optimizer and stabilizer.

Specifications

200×20μl Rxns, 1000×20μl Rxns

Kit components

Part I

Buffer CL

Foregene Protease Plus II

Buffer ST

Part II

DNA Eraser

5× Direct RT Mix

2× Direct qPCR Mix-SYBR

50× ROX Reference Dye

RNase-Free ddH2O

Instructions

Features&advantages

■  Simple and effective : with Cell Direct RT technology, RNA samples can be obtained in just 7 minutes.

■ The sample demand is small, as low as 10 cells can be tested.

■ High throughput: it can quickly detect RNA in cells cultured in 384, 96, 24, 12, 6-well plates.

■ DNA Eraser can quickly remove released genomes, greatly reduce the impact on subsequent experimental results.

■ Optimized RT and qPCR system makes the two-step RT-PCR reverse transcription more efficient and PCR more specific, and more resistant to RT-qPCR reaction inhibitors.

Kit application

Scope of application: cultured cells.

- RNA released by sample lysis: only applicable to the RT-qPCR template of this kit.

- The kit can be used for the following purposes: gene expression analysis, verification of siRNA-mediated gene silencing effect, drug screening, etc.

Diagram

Cell Direct RT qPCR diagram

Storage and Shelf life

Part I of this kit should be stored at 4℃; Part II should be stored at -20℃.

 Foregene Protease Plus II should be stored at 4℃,do not freeze at -20℃.

 Reagent 2×Direct qPCR Mix-SYBR should be stored at -20in the dark; if used frequently, it can also be stored at 4℃ for short-term storage (use up within 10 days).


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