Factory source Animal Tissue Direct Pcr Kit Manual - Mouse Tail Direct PCR Kit-UNG – Foregene
Factory source Animal Tissue Direct Pcr Kit Manual - Mouse Tail Direct PCR Kit-UNG – Foregene Detail:
Specifications
50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns
Kit components
Part I |
Buffer MP |
Foregene Protease Plus |
|
6× DNA Loading Buffer |
|
Part II |
2× M1 PCR EasyTM Mix(UNG) |
Instructions |
Features&advantages
■ No time-consuming and expensive DNA purification is required.
■ The sample demand is small, as little as 5mg mouse tissue or 2-5mm mouse tail can be tested.
■ No special treatments such as grinding and crushing are required, and the operation is simple.
■ The optimized PCR system makes PCR have higher specificity and stronger tolerance to PCR reaction inhibitors.
■ Anti-pollution PCR system 2×M1-PCR EasyTM Mix (UNG), which effectively eliminates the pollution caused by PCR products and ensures the specificity and accuracy of amplification.
Kit parameters
Scope of application: mouse tail, mouse ear, muscle and other tissues.
DNA released from sample lysis: only used as a PCR template.
The kit can be used for the following purposes: identification of transgenosis, animal genotyping, etc.
Work flow
Storage and Shelf life
Part I of this kit should be stored at 2-8℃.
The reagent Buffer AL can be stored for 12 months under dry conditions; it can be stored at 2-8℃ for longer storage.
Reagent Foregene Protease has a unique formula. To ensure its activity and stability, please store it at 4℃ for 12 months.
Reagent 6×DNA Loading Buffer can be stored at 4℃ or -20℃ for a long time.
Part II of this kit should be stored at -20℃.
Reagent 2×M1 PCR EasyTM Mix(UNG), if is used frequently, it can also be stored at 4℃ for short-term storage (use up within 10 days).
Product detail pictures:
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