To become the stage of realizing dreams of our employees! To build a happier, additional united and additional experienced team! To reach a mutual gain of our clients, suppliers, the society and ourselves for Factory supplied China Disinfectant Ethylene Oxide Sterilizer Machine Eto Gas Sterilizer Chamber for Surgical Gown Masks, Together with our efforts, our products and solutions have won the trust of clients and been very salable each here and abroad.
To become the stage of realizing dreams of our employees! To build a happier, additional united and additional experienced team! To reach a mutual gain of our clients, suppliers, the society and ourselves for China Ethylene Oxide Sterilization, Ethylene Oxide Sterilizer, Now we have been sincerely consider to grant brand agent in different areas and our agents’ maximum margin of profit is the most important thing we care about. Welcome all of the friends and customers to join us. We’ve been ready to share win-win corporation.

Kits Descriptions

50 Preps, 200 Preps

This kit uses the spin column and formula developed by our company, which can extract high-purity and high-quality total RNA from various animal tissues with high efficiency.It provides an efficient DNA-Cleaning Column, which can easily separate and adsorb genomic DNA from the supernatant and tissue lysate, simple and time-saving; RNA-only Column can efficiently bind RNA and can be processed simultaneously with a unique formula Lots of samples.

The whole system is RNase-Free, so that the extracted RNA is not degraded; Buffer RW1, Buffer RW2 buffer washing system, so that the obtained RNA is free of protein, DNA, ion, and organic compound pollution.

Kit components:

Features&advantages

■ No need to worry about RNA degradation; the whole system is RNase-Free
■ Effectively remove DNA-using DNA-Cleaning Column
■ Remove DNA without adding DNase
■ Simple-all operations are completed at room temperature
■ Fast -operation can be completed in 30 minutes
■ Safe-no organic reagent required
■ High purity -OD260/280≈1.8-2.1

Kit application

It is suitable for the extraction and purification of total RNA from a variety of fresh or frozen animal tissues or cultured cells.

Product parameters

■ Downstream applications: first-strand cDNA synthesis, RT-PCR, molecular cloning, Northern Blot, etc.
■ Samples: animal tissues, cultured cells
■ Dosage: Tissues 10-20mg, Cells(2-5)×10⁶
■ Maximum DNA binding capacity of purification column: 80 μg
■ Elution volume: 50-200 μl

Work flow

Diagram

Animal Total RNA Isolation Kit treated 20mg
Fresh mouse samples, take 5% purified total RNA 1% agar

Glycogel electrophoresis
1: Spleen 2: Kidney
3: Liver 4: Heart

Storage and shelf life

The kit can be stored for 24 months at room temperature (15–25 ℃) or 2–8 ℃ for longer time. Buffer RL1 can be stored at 4 ℃ for 1 month after adding β- mercaptoethanol (optional).

To become the stage of realizing dreams of our employees! To build a happier, additional united and additional experienced team! To reach a mutual gain of our clients, suppliers, the society and ourselves for Factory supplied China Disinfectant Ethylene Oxide Sterilizer Machine Eto Gas Sterilizer Chamber for Surgical Gown Masks, Together with our efforts, our products and solutions have won the trust of clients and been very salable each here and abroad.
Factory supplied China Ethylene Oxide Sterilization, Ethylene Oxide Sterilizer, Now we have been sincerely consider to grant brand agent in different areas and our agents’ maximum margin of profit is the most important thing we care about. Welcome all of the friends and customers to join us. We’ve been ready to share win-win corporation.