Specifications

200 ×20μl rxns, 2000 ×20μl rxns

2× SuperEasy^TM Mix(UNG)-EDTA :On the basis of 2×SuperEasy^TM Mix-EDTA, dUTP is used instead of dTTP, and the UNG enzyme that can degrade the template containing dUTP is added at the same time, which can effectively prevent the contamination of PCR amplification products. Contains the specially modified D-Taq DNA Polymerase, dNTPs, MgCl2, reaction buffer, PCR reaction enhancer, optimizer and stabilizer, etc. In the PCR reaction, just add the appropriate anticoagulated whole blood, primers, and ddH₂O to the corresponding 2× SuperEasy^TM Mix(UNG)-EDTA to be used in the PCR reaction.

6× DNA Loading Buffer：The Loading Buffer does not contain SDS. It is recommended to use the 6× DNA Loading Buffer included with the kit when performing agarose gel electrophoresis in order to obtain good electrophoresis results. Do not use Loading Buffer containing SDS, otherwise there will be a large tail of bright light in the lane during electrophoresis, which will affect the experimental results.

Kit components

Features&advantages

- There is no need for time-consuming and expensive DNA purification, no pretreatment, and direct use of whole blood as a PCR template.

-The system has strong amplification ability and can sensitively detect the genome and foreign target DNA fragments in the blood.

-2× SuperEasy^TM Mix(UNG)-EDTA has high blood tolerance: up to 45% in human blood and 20% in mouse blood.

-The sample is fully enclosed, so there is no need to worry about sample contamination and false positive PCR results. Anti-pollution PCR system 2×SuperEasy^TM Mix(UNG)-EDTA, effectively eliminates the pollution caused by PCR products, and ensures the specificity and accuracy of amplification.

Kit application

Dedicated to direct PCR identification of EDTA anticoagulated whole blood. (Including: human blood, mouse blood, chicken blood, bird blood, cow blood, dog blood, etc.)

Workflow

Storage and Shelf life

This kit is stored at -20°C.

2× SuperEasy^TM Mix(UNG)-EDTA，If used frequently, it can also be stored at 4°C for short-term storage (use up within 10 days).

6× DNA Loading Buffer,It can be stored at 4°C or -20°C for a long time.