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High Quality for China PCR Sample Preparation Automatic Nucleic Acid Purification Extractor

Kit Description:

This kit uses Real-time RT PCR technology(rRT-PCR) for qualitative detection of SARS-CoV-2 (ORF1ab gene and N gene) nucleic acids in human nasopharyngeal or oropharyngeal swab samples, by multiplex PCR fluorescent probe method.This kit contains an internal control to assess specimen quality, thus no need for nucleic acid purification, can complete test in 1 hour, especially suitable for large-scale rapid detection.


Product Detail

Product Tags

Gaining purchaser fulfillment is our company’s purpose without end. We will make terrific initiatives to acquire new and top-quality solutions, meet up with your exclusive specifications and provide you with pre-sale, on-sale and after-sale providers for High Quality for China PCR Sample Preparation Automatic Nucleic Acid Purification Extractor, High quality, timely services and Aggressive selling price, all acquire us a superior fame in xxx field despite the international intense competition.
Gaining purchaser fulfillment is our company’s purpose without end. We will make terrific initiatives to acquire new and top-quality solutions, meet up with your exclusive specifications and provide you with pre-sale, on-sale and after-sale providers for China Infrared Thermometer, Life-Science Instrument, we have now 8 years experience of production and 5 years experience in trading with the customers all over the world. our clients mainly distributed in the North America, Africa and Eastern Europe. we can supply high quality products with the very competitive price.


Specifications

48 Rxns, 96 Rxns

No.

Component

Amount

Main components

48 Rxns

96 Rxns

1 Nucleic acid release agent 1.4mL/tube 2 tubes 5.3 mL/bottle 1 bottle Surfactant
2 RNA protectant 27 μL/tube 1 tube 53 μL/tube 1 tube RNase inhibitor
3 SARS-CoV-2 reaction solution 800 μL/tube 1 tube 1600 μL/tube 1 tube Primer, probe, reaction buffer, dNTP
4 SARS-CoV-2 enzyme mixture 80 μL/tube 1 tube 160 μL/tube 1 tube Hot start Taq enzyme, M-MLV enzyme
5 SARS-CoV-2 positive control 100 μL/tube 1 tube 100 μL/tube 1 tube Recombinant plasmid containing target fragment, RNA
6 SARS-CoV-2 negative control 1200 μL/tube 1 tube 1200 μL/tube 1 tube TE buffer

Features&advantages

Quick and easy.
Avoid nucleic acid loss.
Low detection limit and high sensitivity.
Low equipment requirement and highly flexible use.

Detection sensitivity can reach the lowest level as 500 copies/mL).

GCE/mL Ct value
N gene ORF1ab gene
2.00E+08 16.43 15.16
2.00E+07 19.48 18.58
2.00E+06 22.71 21.86
2.00E+05 25.95 25.33
2.00E+04 29.30 28.49
2.00E+03 32.37 31.37
2.00E+02 35.32 34.47
2.00E+01 38.29 38.27
2.00E+00 N/A N/A
2.00E-01 N/A N/A
NC N/A N/A

Linear amplification curve

amplification curve-RT-qPCR

How to use

1. Applicable instrument

covid-19 nucleic acid detection kit1

2. Detection procedure

covid-19 nucleic acid detection kit2

Sample processing:Naopharyngeal or oropharyngeal swab sample (only 1 step operation)(Wait for 10 mins)

PCR amplificaton(<=55min)

Workflow:

covid-19 nucleic acid detection kit4

Storage

sealed from light and stored at -20±5℃;

storage
storage2

Shelf life: 1 year

Gaining purchaser fulfillment is our company’s purpose without end. We will make terrific initiatives to acquire new and top-quality solutions, meet up with your exclusive specifications and provide you with pre-sale, on-sale and after-sale providers for High Quality for China PCR Sample Preparation Automatic Nucleic Acid Purification Extractor, High quality, timely services and Aggressive selling price, all acquire us a superior fame in xxx field despite the international intense competition.
High Quality for China Infrared Thermometer, Life-Science Instrument, we have now 8 years experience of production and 5 years experience in trading with the customers all over the world. our clients mainly distributed in the North America, Africa and Eastern Europe. we can supply high quality products with the very competitive price.


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